Isolation and characterization of microsatellite loci in Colletotrichum acutatum, the causal agent of postbloom fruit drop on citrus

Author(s)

Ciampi, M. B. ^{[1, 2]} ; Baldauf, C. ^{[3, 1]} ; Vigna, B. B. Z. ^[1] ; Souza, A. P. ^{[3, 1]} ; Sposito, M. B. ^[4] ; Amorim, L. ^[2]

Affiliation

^[1] Univ Estadual Campinas, Genet Engn & Mol Biol Ctr, BR-13083875 Campinas, SP - Brazil

^[2] Univ Sao Paulo, Dept Plant Pathol, ESALQ, BR-13418900 Piracicaba, SP - Brazil

^[3] Univ Estadual Campinas, Dept Plant Biol, BR-13083970 Campinas, SP - Brazil

^[4] Univ Sao Paulo, Dept Crop Sci, ESALQ, BR-13418900 Piracicaba, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 3, n. 4, p. 651-654, OCT 2011.
• Web of Science Citations: 7

Abstract

From a genomic enriched library, we developed 27 primer pairs from microsatellite flanking sequences for Colletotrichum acutatum, associated to postbloom fruit drop disease on citrus. Loci were characterized using 40 monosporic C. acutatum isolates. Nine primer pairs successfully amplified polymorphic microsatellite regions, with 3-6 alleles per locus, and mean heterozygosities ranging 0.093-0.590 across loci. The suitability of these primers was investigated in four Colletotrichum species as well. These microsatellite markers will be useful for genetic analyses and epidemiological studies of C. acutatum. (AU)

• FAPESP's process: 08/54176-4 - Molecular epidemiology and management of postbloom fruit drop of citrus in new planting areas in São Paulo State
• Grantee: Lilian Amorim
• Support Opportunities: Research Projects - Thematic Grants