Poly I:C elicits broader and stronger humoral and cellular responses to a Plasmodium vivax circumsporozoite protein malaria vaccine than Alhydrogel in mice

Author(s):Show less -

Costa-Gouvea, Tiffany B. L. ; Francoso, Katia S. ; Marques, Rodolfo F. ; Gimenez, Alba Marina ; Faria, Ana C. M. ; Cariste, Leonardo M. ; Dominguez, Mariana R. ; Vasconcelos, Jose Ronnie C. ; Nakaya, Helder I. ; Silveira, Eduardo L. V. ; Soares, Irene S.

• Document type: Journal article
• Source: FRONTIERS IN IMMUNOLOGY; v. 15, p. 15-pg., 2024-04-08.

Abstract

Malaria remains a global health challenge, necessitating the development of effective vaccines. The RTS,S vaccination prevents Plasmodium falciparum (Pf) malaria but is ineffective against Plasmodium vivax (Pv) disease. Herein, we evaluated the murine immunogenicity of a recombinant PvCSP incorporating prevalent polymorphisms, adjuvanted with Alhydrogel or Poly I:C. Both formulations induced prolonged IgG responses, with IgG1 dominance by the Alhydrogel group and high titers of all IgG isotypes by the Poly I:C counterpart. Poly I:C-adjuvanted vaccination increased splenic plasma cells, terminally-differentiated memory cells (MBCs), and precursors relative to the Alhydrogel-combined immunization. Splenic B-cells from Poly I:C-vaccinated mice revealed an antibody-secreting cell- and MBC-differentiating gene expression profile. Biological processes such as antibody folding and secretion were highlighted by the Poly I:C-adjuvanted vaccination. These findings underscore the potential of Poly I:C to strengthen immune responses against Pv malaria. (AU)

• FAPESP's process: 19/06494-1 - Comparative analysis between the humoral and cellular immune responses elicited by a malaria vaccine adjuvanted with poly I:C or alum in C57BL/6 mice
• Grantee: Tiffany Bianca Lage da Costa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/18102-7 - Preclinical tests of recombinant adenovirus-based protein and vaccine formulations expressing the Plasmodium vivax circumsporozoite protein
• Grantee: Alba Marina Gimenez
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/14933-2 - Integrative biology applied to human health
• Grantee: Helder Takashi Imoto Nakaya
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 17/11931-6 - Identification of B cell protective antigens/epitopes against asexual and sexual blood forms of Plasmodium parasites through monoclonal antibodies
• Grantee: Eduardo Lani Volpe da Silveira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/25373-0 - Influence of the proteic-energetic malnutrution in the humoral e cellular immune responses elicited by a malaria vaccine in C57Bl/6 mice
• Grantee: Ana Carolina Machado de Faria
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/13032-5 - Generation and analysis of the immunogenicity of recombinant proteins based on the different allelic forms of the circumsporozoite antigen of Plasmodium vivax aiming at the development of a universal vaccine against malaria
• Grantee: Irene da Silva Soares
• Support Opportunities: Research Projects - Thematic Grants