The role of satellite DNAs in the chromosomal rearrangements and the evolution of the rare XY₁Y₂ sex system in Harttia (Siluriformes: Loricariidae)

Author(s)

Deon, Geize Aparecida ; dos Santos, Rodrigo Zeni ; Sassi, Francisco de Menezes Cavalcante ; Moreira-Filho, Orlando ; Vicari, Marcelo Ricardo ; Porto-Foresti, Fabio ; Utsunomia, Ricardo ; Cioffi, Marcelo de Bello

• Document type: Journal article
• Source: JOURNAL OF HEREDITY; v. 115, n. 5, p. 11-pg., 2024-05-17.

Abstract

The underlying processes behind the formation, evolution, and long-term maintenance of multiple sex chromosomes have been largely neglected. Among vertebrates, fishes represent the group with the highest diversity of multiple sex chromosome systems and, with six instances, the Neotropical fish genus Harttia stands out by presenting the most remarkable diversity. However, although the origin mechanism of their sex chromosome systems is well discussed, little is known about the importance of some repetitive DNA classes in the differentiation of multiple systems. In this work, by employing a combination of cytogenetic and genomic procedures, we evaluated the satellite DNA composition of H. carvalhoi with a focus on their role in the evolution, structure, and differentiation process of the rare XY1Y2 multiple-sex chromosome system. The genome of H. carvalhoi contains a total of 28 satellite DNA families, with the A + T content ranging between 38.1% and 68.1% and the predominant presence of long satellites. The in situ hybridization experiments detected 15 satellite DNAs with positive hybridization signals mainly on centromeric and pericentromeric regions of almost all chromosomes or clustered on a few pairs. Five of them presented clusters on X, Y-1,Y- and/or Y-2 sex chromosomes which were therefore selected for comparative hybridization in the other three congeneric species. We found several conserved satellites accumulated on sex chromosomes and also in regions that were involved in chromosomal rearrangements. Our results provide a new contribution of satellitome studies in multiple sex chromosome systems in fishes and represent the first satellitome study for a Siluriformes species. (AU)

• FAPESP's process: 23/00955-2 - Integrating cytogenetics and genomics in the study of sex chromosome differentiation pathways. Part I
• Grantee: Marcelo de Bello Cioffi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/13381-1 - EVOLUTION OF CATALOGUES OF SATELLITE DNAs IN CHARACIFORMES (Vertebrata: Teleostei): A 100 MILLION YEARS OF HISTORY
• Grantee: Ricardo Utsunomia
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/14096-6 - Evolutionary history of the rare multiple XY1Y2 sex chromosome system. Satellitome analysis in fish and mammal species
• Grantee: Geize Aparecida Deon
• Support Opportunities: Scholarships in Brazil - Post-Doctoral