Advanced search
Start date
Betweenand


Dry blood spots as a sampling strategy to identify insulin resistance markers during a dietary challenge

Full text
Author(s):
Duarte, Stephany Goncalves ; Donado-Pestana, Carlos M. ; More, Tushar H. ; Rodrigues, Larissa ; Hiller, Karsten ; Fiamoncini, Jarlei
Total Authors: 6
Document type: Journal article
Source: GENES AND NUTRITION; v. 19, n. 1, p. 9-pg., 2024-12-01.
Abstract

This study aimed to identify markers of postprandial dysglycemia in the blood of self-described healthy individuals using dry blood spots (DBS) as a sampling strategy. A total of 54 volunteers, including 31 women, participated in a dietary challenge. They consumed a high-fat, high-sugar mixed meal and underwent multiple blood sampling over the course of 150 min to track their postprandial responses. Blood glucose levels were monitored with a portable glucometer and individuals were classified into two groups based on the glucose area under the curve (AUC): High-AUC (H-AUC) and Low-AUC (L-AUC). DBS sampling was performed at the same time points as the assessment of glycemia using Whatman 903 Protein Saver filter paper. A gas chromatography-mass spectrometry-based metabolite profiling was conducted in the DBS samples to assess postprandial changes in blood metabolome. Higher concentrations of metabolites associated with insulin resistance were observed in individuals from the H-AUC group, including sugars and sugar-derived products such as fructose and threonic acid, as well as organic acids and fatty acids such as succinate and stearic acid. Several metabolites detected in the GC-MS analysis remained unidentified, indicating that other markers of hyperglycemia remain to be discovered in DBS. Based on these observations, we demonstrated that the use of DBS as a non-invasive and inexpensive sampling tool allows the identification of metabolites markers of dysglycemia in the postprandial period. (AU)

FAPESP's process: 21/09237-0 - Postprandial metabolism and inflammation: the role of bile acids as signaling molecules
Grantee:Larissa Rodrigues
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 20/16542-0 - Relationship between inflammation and primary metabolism in the postprandial period
Grantee:Carlos Mario Donado Pestana
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 22/02941-6 - Bile acids as determinants of postprandial metabolism and intestinal microbial colonization in newborns
Grantee:Jarlei Fiamoncini
Support Opportunities: Research Grants - Initial Project
FAPESP's process: 21/08657-5 - Evaluation of D-limonene effects on postprandial intermediary metabolism using "dry blood spots" as a sampling tool in a clinical study carried out remotely
Grantee:Jarlei Fiamoncini
Support Opportunities: Regular Research Grants