Ascorbic acid as a modulator of inflammatory response against Candida albicans

Author(s)

Moreira Milhan, Noala Vicensoto ; Graca Sampaio, Aline da ; Koga-Ito, Cristiane Yumi ; Bruzzaniti, Angela

• Document type: Journal article
• Source: FUTURE MICROBIOLOGY; v. 19, n. 7, p. 10-pg., 2024-04-17.

Abstract

Aim: To evaluate the behavior of oral keratinocytes in the presence of Vitamin C (Vit C) and its anti-inflammatory potential. Materials & methods: Oral keratinocytes were initially exposed to 0.1-2.5 mM of Vit C and the metabolic activity and cell migration were evaluated using MTS assay and Ibidi culture inserts, respectively. After, the cells were challenged with Candida albicans and inflammatory markers were analyzed by qPCR. Results: The treatment was not cytotoxic, and the highest concentrations increased the metabolic activity at 24 h. Vit C delayed the cell migration at 48 and 72 h. Interestingly, it downregulated the genes IL-8 and IL-1 beta. Conclusion: Vit C could be an interesting adjuvant to anti-fungal treatment due to its anti-inflammatory potential. (AU)

• FAPESP's process: 22/04723-6 - Application of low temperature cold plasma in the treatment of mucositis using an organotypic 3D model
• Grantee: Aline da Graça Sampaio
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 22/04598-7 - The effect of synergistic treatment of vitamin C and low temperature plasma on candidiasis induced in reconstituted oral mucosa
• Grantee: Noala Vicensoto Moreira Milhan
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 19/05856-7 - Use of low temperature atmospheric pressure plasma in dentistry: from laboratory bench to clinics
• Grantee: Cristiane Yumi Koga Ito
• Support Opportunities: Research Projects - Thematic Grants