| Full text | |
| Author(s): Show less - |
Prado, Guilherme Souza
;
Rocha, Dhiovanna Correia
;
dos Santos, Lucas Nascimento
;
Contiliani, Danyel Fernandes
;
Nobile, Paula Macedo
;
Martinati-Schenk, Juliana Camargo
;
Padilha, Lilian
;
Maluf, Mirian Perez
;
Lubini, Greice
;
Pereira, Tiago Campos
;
Monteiro-Vitorello, Claudia Barros
;
Creste, Silvana
;
Boscariol-Camargo, Raquel Luciana
;
Takita, Marco Aurelio
;
Cristofani-Yaly, Mariangela
;
de Souza, Alessandra Alves
Total Authors: 16
|
| Document type: | Journal article |
| Source: | FRONTIERS IN PLANT SCIENCE; v. 14, p. 27-pg., 2024-01-08. |
| Abstract | |
Gene editing technologies have opened up the possibility of manipulating the genome of any organism in a predicted way. CRISPR technology is the most used genome editing tool and, in agriculture, it has allowed the expansion of possibilities in plant biotechnology, such as gene knockout or knock-in, transcriptional regulation, epigenetic modification, base editing, RNA editing, prime editing, and nucleic acid probing or detection. This technology mostly depends on in vitro tissue culture and genetic transformation/transfection protocols, which sometimes become the major challenges for its application in different crops. Agrobacterium-mediated transformation, biolistics, plasmid or RNP (ribonucleoprotein) transfection of protoplasts are some of the commonly used CRISPR delivery methods, but they depend on the genotype and target gene for efficient editing. The choice of the CRISPR system (Cas9, Cas12), CRISPR mechanism (plasmid or RNP) and transfection technique (Agrobacterium spp., PEG solution, lipofection) directly impacts the transformation efficiency and/or editing rate. Besides, CRISPR/Cas technology has made countries rethink regulatory frameworks concerning genetically modified organisms and flexibilize regulatory obstacles for edited plants. Here we present an overview of the state-of-the-art of CRISPR technology applied to three important crops worldwide (citrus, coffee and sugarcane), considering the biological, methodological, and regulatory aspects of its application. In addition, we provide perspectives on recently developed CRISPR tools and promising applications for each of these crops, thus highlighting the usefulness of gene editing to develop novel cultivars. (AU) | |
| FAPESP's process: | 20/07045-3 - Biotechnological and genomic strategies for quality, productivity and sustainable management of citrus, coffee and sugar cane in the State of São Paulo |
| Grantee: | Mariângela Cristofani-Yaly |
| Support Opportunities: | Research Grants - Problem-Oriented Research Centers in São Paulo |
| FAPESP's process: | 21/13478-2 - Genome editing of grass species via CRISPR/Cas9 |
| Grantee: | Danyel Fernandes Contiliani |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 22/10505-1 - Genetic Engineering and Synthetic Biology approaches applied to the development of novel resistant or tolerant sweet orange (Citrus sinensis) varieties against Huanglongbing (HLB) |
| Grantee: | Guilherme Souza Prado |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 22/11738-0 - Plant genome engineering with CRISPR-dCas12b system |
| Grantee: | Danyel Fernandes Contiliani |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |