In vitro effect of TiF₄/NaF solution on the development of radiation-induced dentin caries

Author(s)

de Souza, Beatriz Martines ; Francisco, Eduardo Lista ; Braga, Aline Silva ; Santos, Paulo Sergio da Silva ; Buzalaf, Marilia Afonso Rabelo ; Magalhaes, Ana Carolina

• Document type: Journal article
• Source: Journal of Applied Oral Science; v. 32, p. 7-pg., 2024-01-01.

Abstract

Objective: To evaluate the protective effect of an experimental solution containing TiF4/NaF on the development of radiation-induced dentin caries lesions. Methodology: bovine root samples were irradiated (70Gy) and distributed as following (n=12/group): Commercial Saliva (BioXtra), NaF (500 ppm F-), TiF4 (500 ppm F), TiF4/NaF (TiF4: 300 ppm F-, NaF: 190 ppm F-), and Phosphate buffer solution (PBS, negative control). Biofilm was produced using biofilm from irradiated patients and McBain saliva (0.2% of sucrose, at 37oC and 5% CO2) for five days. The treatments were applied 1x/day. Colony-forming units (CFU) were counted and demineralization was quantified by transversal microradiography. The ANOVA/Tukey test was applied for all parameters. Results: All treatments reduced CFU for total microorganisms. TiF4 reduced Lactobacillus sp. (7.04 +/- 0.26 log10 CFU/ mL) and mutans streptococci (7.18 +/- 0.28) CFU the most, when compared to PBS (7.58 +/- 0.21 and 7.75 +/- 0.17) and followed by NaF (7.12 +/- 0.31 and 7.34 +/- 0.22) and TiF4/NaF (7.16 +/- 0.35 and 7.29 +/- 0.29). TiF4 and Commercial saliva showed the lowest integrated mineral loss (Delta Z-vol%.mm) (1977 +/- 150 and 2062 +/- 243, respectively) when compared to PBS (4540 +/- 335), followed by NaF (2403 +/- 235) and TiF4/NaF (2340 +/- 200). Commercial saliva was the only to significantly reduce mineral loss (LD-mu m) (111 +/- 25) compared to PBS (153 +/- 24).Mean mineral loss (R-vol%) decreased by 35.2% for TiF4 (18.2 +/- 3.3) when compared to PBS (28.1 +/- 2.9) Conclusion: TiF4/NaF has a comparable anti-cariogenic effect to TiF4 and Commercial saliva under the model in this study. (AU)

• FAPESP's process: 19/07241-0 - Effect of artificial saliva substitutes and fluoride application on the development of root caries under a microcosm biofilm model simulating patients subjected to head and neck radiation
• Grantee: Beatriz Martines de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/21797-0 - Effect of artificial saliva substitutes and fluoride application on the development of root caries under a microcosm biofilm model simulating patients subjected to head and neck radiation
• Grantee: Ana Carolina Magalhães
• Support Opportunities: Regular Research Grants