Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intraspecific sequence variation in 16S rRNA gene of Ureaplasma diversum isolates

Full text
Author(s):
Marques, L. M. [1, 2] ; Buzinhani, M. [1] ; Guimaraes, A. M. S. [1] ; Marques, R. C. P. [1] ; Farias, S. T. [3] ; Neto, R. L. [1] ; Yamaguti, M. [1] ; Oliveira, R. C. [1] ; Timenetsky, J. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05508900 Sao Paulo - Brazil
[2] Univ Fed Bahia, Inst Multidisciplinar Saude, Nucleo Tecnol Saude, BR-41170290 Salvador, BA - Brazil
[3] Univ Fed Paraiba, Ctr Ciencias Exatas & Nat, Dept Biol Mol, BR-58059900 Joao Pessoa, Paraiba - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Veterinary Microbiology; v. 152, n. 1-2, p. 205-211, AUG 26 2011.
Web of Science Citations: 5
Abstract

Ureaplasma diversum infection in bulls may result in seminal vesiculitis, balanoposthitis and alterations in spermatozoids. In cows, it can cause placentitis, fetal alveolitis, abortion and the birth of weak calves. U. diversum ATCC 49782 (serogroups A), ATCC 49783 (serogroup C) and 34 field isolates were used for this study. These microorganisms were submitted to Polymerase Chain Reaction for 16S gene sequence determination using Tact High Fidelity and the products were purified and bi-directionally sequenced. Using the sequence obtained, a fragment containing four hypervariable regions was selected and nucleotide polymorphisms were identified based on their position within the 16S rRNA gene. Forty-four single nucleotide polymorphisms (SNP) were detected. The genotypic variability of the 16S rRNA gene of U. diversum isolates shows that the taxonomy classification of these organisms is likely much more complex than previously described and that 16S rRNA gene sequencing may be used to suggest an epidemiologic pattern of different origin strains. (c) 2011 Elsevier B.V. All rights reserved. (AU)