Spontaneous running wheel exercise during pregnancy prevents later neonatal-anoxia-induced somatic and neurodevelopmental alterations

Author(s)

Lee, Vitor Yonamine ; Nils, Aline Vilar Machado ; Arruda, Bruna Petrucelli ; Xavier, Gilberto Fernando ; Nogueira, Maria Ines ; Motta-Teixeira, Livia Clemente ; Takada, Silvia Honda

• Document type: Journal article
• Source: IBRO NEUROSCIENCE REPORTS; v. 17, p. 17-pg., 2024-09-09.

Abstract

Introduction: About 15-20 % of babies that suffer perinatal asphyxia die and around 25 % of the survivors exhibit permanent neural outcomes. Minimization of this global health problem has been warranted. This study investigated if the offspring of pregnant female rats allowed to spontaneously exercise on running wheels along a 11-day pregnancy period were protected for somatic and neurodevelopmental disturbs that usually follow neonatal anoxia. Methods: spontaneous exercise was applied to female rats which were housed in cages allowing free access to running wheels along a 11-day pregnancy period. Their offspring were submitted to anoxia 24-36 h after birth. Somatic and sensory-motor development of the pups were recorded until postnatal day 21 (P21). Myelin basic protein (MBP)-stained areas of sensory and motor cortices were measured at P21. Neuronal nuclei (NeuN)immunopositive cells and synapsin-I levels in hippocampal formation were estimated at P21 and P75. Results: gestational exercise and / or neonatal anoxia increased the weight and the size of the pups. In addition, gestational exercise accelerated somatic and sensory-motor development of the pups and protected them against neonatal-anoxia-induced delay in development. Further, neonatal anoxia reduced MBP stained area in the secondary motor cortex and decreased hippocampal neuronal estimates and synapsin-I levels at P21; gestational exercise prevented these effects. Therefore, spontaneous exercise along pregnancy is a valuable strategy to prevent neonatal-anoxia-induced disturbs in the offspring. Conclusion: spontaneous gestational running wheel exercise protects against neonatal anoxia-induced disturbs in the offspring, including (1) physical and neurobehavioral developmental impairments, and (2) hippocampal and cortical changes. Thus, spontaneous exercise during pregnancy may represent a valuable strategy to prevent disturbs which usually follow neonatal anoxia. (AU)

• FAPESP's process: 11/03960-0 - Physical activity, neurogenesis and memory
• Grantee: Lívia Clemente Motta Teixeira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 19/17239-2 - Adult Neurogenesis during Lactation and Maternal Behavior: Implications of melanin-concentrating hormone (MCH) and Melatonin
• Grantee: Lívia Clemente Motta Teixeira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/19747-3 - Organization and ultrastructure of 5-bromo-2'-deoxiuridine (BrdU)-immunoreactive cells in neurogenic niche of lateral ventricle in Long-Evans rats (Rattus norvegicus)
• Grantee: Maria Inês Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 10/01488-9 - Study of the organization and of the neuronal dynamics: neuroanal death and neurogenesis of the rats brain after neonatal anoxia
• Grantee: Maria Inês Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/14779-1 - Perinatal asphyxia and use of antioxidant agents as neuroprotectors: relevance to Alzheimer's disease
• Grantee: Silvia Honda Takada
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/16268-6 - Impact of neuroinflammation and the participation of the gut-brain axis in myelination in a rodent model of neonatal anoxia combined with perinatal inflammation by maternal immune activation
• Grantee: Silvia Honda Takada
• Support Opportunities: Regular Research Grants