Five Weeks of Sprint Interval Training Improve Muscle Glycolytic Content and Activity But Not Time to Task Failure in Severe-Intensity Exercise

Author(s)

De Poli, Rodrigo Araujo Bonetti ; Murias, Juan Manuel ; Antunes, Barbara Moura ; Marinari, Gabriele ; Dutra, Yago Medeiros ; Milioni, Fabio ; Zagatto, Alessandro Moura

• Document type: Journal article
• Source: MEDICINE AND SCIENCE IN SPORTS AND EXERCISE; v. 56, n. 8, p. 13-pg., 2024-08-01.

Abstract

PurposeThis study examined the impact of a 5-wk sprint interval training (SIT) intervention on time to task failure (TTF) during severe-intensity constant work rate (CWR) exercise, as well as in glycolytic enzymatic content and activity, and glycogen content.MethodsFourteen active males were randomized into either a SIT group (n = 8) composed of 15 SIT sessions over 5 wk, or a control group (n = 6). At pretraining period, participants performed i) ramp incremental test to measure the cardiorespiratory function; ii) CWR cycling TTF at 150% of the power output (PO) at the respiratory compensation point (RCP-PO) with muscle biopsies at rest and immediately following task failure. After 5 wk, the same evaluations were repeated (i.e., exercise intensities matched to current training status), and an additional cycling CWR matched to pretraining 150% RCP-PO was performed only for TTF evaluation. The content and enzymatic activity of glycogen phosphorylase (GPhos), hexokinase (HK), phosphofructokinase (PFK), and lactate dehydrogenase (LDH), as well as the glycogen content, were analyzed. Content of monocarboxylate transporter isoform 4 (MCT4) and muscle buffering capacity were also measured.ResultsDespite improvements in total work performed at CWR posttraining, no differences were observed for TTF. The GPhos, HK, PFK, and LDH content and activity, and glycogen content also improved after training only in the SIT group. Furthermore, the MCT4 concentrations and muscle buffering capacity were also improved only for the SIT group. However, no difference in glycogen depletion was observed between groups and time.ConclusionsFive weeks of SIT improved the glycolytic pathway parameters and total work performed; however, glycogen depletion was not altered during CWR severe-intensity exercise, and TTF remained similar. (AU)

• FAPESP's process: 18/21126-6 - Fatigue process during supramaximal effort: Analysis of central and peripherical mechanisms, muscular acidosis, energetic substrate, enzymatic activity and metabolic
• Grantee: Alessandro Moura Zagatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/17445-1 - Influence of high intensity interval training on time to exhaustion during a supramaximal effort: biomolecular and neuromuscular responses
• Grantee: Rodrigo de Araujo Bonetti de Poli
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/08479-0 - Time course of neuromuscular recovery after running training session: influence of intensity, hot water immersion and cold water immersion
• Grantee: Yago Medeiros Dutra
• Support Opportunities: Scholarships in Brazil - Doctorate