Assessing the negative impact of chlorantraniliprole, isoxaflutole, and simazine pesticides on phospholipid membrane models and tilapia gill tissues

Author(s)

Rubira, Rafael J. G. ; Correia, Rafael R. ; Batista, Victor R. G. ; Pazin, Wallance M. ; Gonzalez, Francisco G. ; Otero, Juan C. ; Teixeira, Giovana R. ; Job, Aldo E.

• Document type: Journal article
• Source: Environmental Pollution; v. 349, p. 13-pg., 2024-04-04.

Abstract

The indiscriminate and, very often, incorrect use of pesticides in Brazil, as well as in other countries, results in severe levels of environmental pollution and intoxication of human life. Herein, we studied plasma membrane models (monolayer and bilayer) of the phospholipid Dioleoyl-sn-glycerol-3-phosphocholine (DOPC) using Langmuir films, and large (LUVs) and giant (GUVs) unilamellar vesicles, to determine the effect of the pesticides chlorantraniliprole (CLTP), isoxaflutole (ISF), and simazine (SMZ), used in sugarcane. CLTP affects the lipid organization of the bioinspired models of DOPC pi-A isotherms, while ISF and SMZ pesticides significantly affect the LUVs and GUVs. Furthermore, the in vivo study of the gill tissue in fish in the presence of pesticides (2.0 x 10-10 mol/L for CLTP, 8.3 x 10-9 mol/L for ISF, and SMZ at 9.9 x 10-9 mol/L) was performed using optical and fluorescence images. This investigation was motivated by the gill lipid membranes, which are vital for regulating transporter activity through transmembrane proteins, crucial for maintaining ionic balance in fish gills. In this way, the presence of phospholipids in gills offers a model for understanding their effects on fish health. Histological results show that exposure to CLTP, ISF, and SMZ may interfere with vital gill functions, leading to respiratory disorders and osmoregulation dysfunction. The results indicate that exposure to pesticides caused severe morphological alterations in fish, which could be correlated with their impact on the bioinspired membrane models. Moreover, the effect does not depend on the exposure period (24h and 96h), showing that animals exposed to pesticides for a short period suffer irreparable damage to gill tissue. In summary, we can conclude that the harm caused by pesticides, both in membrane models and in fish gills, occurs due to contamination of the aquatic system with pesticides. Therefore, water quality is vital for the preservation of ecosystems. (AU)

• FAPESP's process: 21/14514-2 - Analysis of cancer cachexia associated with the treatment of drug-carrying nanoparticles of docetaxel and methotrexate in sprague-dawley rats submitted to aerobic physical training
• Grantee: Rafael Ribeiro Correia
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 20/16310-2 - Development of biotechnology applied to prostatic carcinomatosis with the use of Docetaxel chemotherapy care nanoparticles associated with aerobic physical training
• Grantee: Giovana Rampazzo Teixeira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/05423-0 - Detection of pesticides (cocktails) used in sugarcane crops and their effect on biological systems
• Grantee: Rafael Jesus Gonçalves Rubira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/15324-0 - Effects of high intensity interval physical training (HIIT) on the histological, biochemical and molecular parameters of the urinary bladder of spontaneously hypertensive rats (SHR)
• Grantee: Victor Rogério Garcia Batista
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 21/13884-0 - Analysis of biological systems exposed to pesticides by Coherent Anti-Stokes Raman Spectroscopy (CARS)
• Grantee: Rafael Jesus Gonçalves Rubira
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor