The telocytes relationship with satellite cells: Extracellular vesicles mediate the myotendinous junction remodeling

Author(s)

Pimentel Neto, Jurandyr ; Batista, Rodrigo Daniel ; Rocha-Braga, Lara Caetano ; Chacur, Marucia ; Camargo, Paula Oliveira ; Ciena, Adriano Polican

• Document type: Journal article
• Source: MICROSCOPY RESEARCH AND TECHNIQUE; v. 87, n. 8, p. 9-pg., 2024-03-19.

Abstract

The peripheral nerve injury (PNI) affects the morphology of the whole locomotor apparatus, which can reach the myotendinous junction (MTJ) interface. In the injury condition, the skeletal muscle satellite cells (SC) are triggered, activated, and proliferated to repair their structure, and in the MTJ, the telocytes (TC) are associated to support the interface with the need for remodeling; in that way, these cells can be associated with SC. The study aimed to describe the SC and TC relationship after PNI at the MTJ. Sixteen adult Wistar rats were divided into Control Group (C, n = 8) and PNI Group (PNI, n = 8), PNI was performed by the constriction of the sciatic nerve. The samples were processed for transmission electron microscopy and immunostaining analysis. In the C group was evidenced the arrangement of sarcoplasmic evaginations and invaginations, the support collagen layer with a TC inside it, and an SC through vesicles internally and externally to then. In the PNI group were observed the disarrangement of invaginations and evaginations and sarcomeres degradation at MTJ, as the disposition of telopodes adjacent and in contact to the SC with extracellular vesicles and exosomes in a characterized paracrine activity. These findings can determine a link between the TCs and the SCs at the MTJ remodeling.Research Highlights Peripheral nerve injury promotes the myotendinous junction (MTJ) remodeling. The telocytes (TC) and the satellite cells (SC) are present at the myotendinous interface. TC mediated the SC activity at MTJ. The illustration demonstrates the main purpose of the study of the interaction among satellite cell (SC) and telocytes at the myotendinous junction (MTJ) with the extracellular vesicles contact with and without peripheral nerve injury. Created with BioRender.com. image (AU)

• FAPESP's process: 21/10471-7 - TELOCYTES AND PERISINAPTIC SCHWANN'S CELLS: REPERCUSSION ON THE NEUROMUSCULAR JUNCTION IN AN EXPERIMENTAL MODEL OF MUSCLE INJURY
• Grantee: Jurandyr Pimentel Neto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/04272-4 - Muscle regeneration: association of telocytes and satellite cells in the plasticity of the myotendinous junction
• Grantee: Adriano Polican Ciena
• Support Opportunities: Regular Research Grants