| Full text | |
| Author(s): |
Dalmaso, Barbara
;
Liber, Andre Mauricio Passos
;
Ventura, Dora Fix
;
Jancar, Sonia
;
Del Debbio, Carolina Beltrame
Total Authors: 5
|
| Document type: | Journal article |
| Source: | FRONTIERS IN CELLULAR NEUROSCIENCE; v. 18, p. 17-pg., 2024-03-20. |
| Abstract | |
Introduction Platelet-activating factor (PAF), PAF receptor (PAFR), and PAF- synthesis/degradation systems are involved in essential CNS processes such as neuroblast proliferation, differentiation, migration, and synaptic modulation. The retina is an important central nervous system (CNS) tissue for visual information processing. During retinal development, the balance between Retinal Progenitor Cell (RPC) proliferation and differentiation is crucial for proper cell determination and retinogenesis. Despite its importance in retinal development, the effects of PAFR deletion on RPC dynamics are still unknown.Methods We compared PAFR knockout mice (PAFR-/-) retinal postnatal development proliferation and differentiation aspects with control animals. Electrophysiological responses were analyzed by electroretinography (ERG).Results and discussion In this study, we demonstrate that PAFR-/- mice increased proliferation during postnatal retinogenesis and altered the expression of specific differentiation markers. The retinas of postnatal PAFR-/- animals decreased neuronal differentiation and synaptic transmission markers, leading to differential responses to light stimuli measured by ERG. Our findings suggest that PAFR signaling plays a critical role in regulating postnatal RPC cell differentiation dynamics during retinal development, cell organization, and neuronal circuitry formation. (AU) | |
| FAPESP's process: | 20/11352-9 - The role of PAF receptor in retinal stem cell reprogramming and differentiation |
| Grantee: | Bárbara Dalmaso |
| Support Opportunities: | Scholarships in Brazil - Doctorate (Direct) |
| FAPESP's process: | 15/24001-1 - MicroRNAs role in retinal stem cells regulation |
| Grantee: | Carolina Beltrame Del Debbio |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 19/00777-1 - Relationship between circadian habits and visual functions in diurnal and nocturnal snakes (snakes, Colubridae) |
| Grantee: | André Maurício Passos Liber |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 22/00191-0 - Mechanisms, genetics and performance of the visual system: clinical research in humans and animal experiments |
| Grantee: | Dora Selma Fix Ventura |
| Support Opportunities: | Research Projects - Thematic Grants |