CRISPR/CAS as a Powerful Tool for Human Immunodeficiency Virus Cure: A Review

Author(s)

Komninakis, Shirley Vasconcelos ; Domingues, Wilson ; Sanabani, Sabri Saeed ; Folgosi, Victor Angelo ; Barbosa, Igor Neves ; Casseb, Jorge

• Document type: Journal article
• Source: AIDS Research and Human Retroviruses; v. 40, n. 6, p. 13-pg., 2024-02-02.

Abstract

Despite care and the availability of effective antiretroviral treatment, some human immunodeficiency virus (HIV)-infected individuals suffer from neurocognitive disorders associated with HIV (HAND) that significantly affect their quality of life. The different types of HAND can be divided into asymptomatic neurocognitive impairment, mild neurocognitive disorder, and the most severe form known as HIV-associated dementia. Little is known about the mechanisms of HAND, but it is thought to be related to infection of astrocytes, microglial cells, and macrophages in the human brain. The formation of a viral reservoir that lies dormant as a provirus in resting CD4+ T lymphocytes and in refuge tissues such as the brain contributes significantly to HIV eradication. In recent years, a new set of tools have emerged: the gene editing based on the clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system, which can alter genome segments by insertion, deletion, and replacement and has great therapeutic potential. This technology has been used in research to treat HIV and appears to offer hope for a possible cure for HIV infection and perhaps prevention of HAND. This approach has the potential to directly impact the quality of life of HIV-infected individuals, which is a very important topic to be known and discussed. (AU)

• FAPESP's process: 18/07239-2 - Incidence, risk factors, and pathogenic mechanism of neurocognitive disorders (HAND) among HIV-1-infected subjects
• Grantee: Jorge Simao do Rosario Casseb
• Support Opportunities: Research Projects - Thematic Grants