| Full text | |
| Author(s): |
Valente, Leticia Cardoso
;
Bacil, Gabriel Prata
;
Riechelmann-Casarin, Luana
;
Barbosa, Giullia Cavichiolli
;
Barbisan, Luis Fernando
;
Romualdo, Guilherme Ribeiro
Total Authors: 6
|
| Document type: | Journal article |
| Source: | Life Sciences; v. 351, p. 13-pg., 2024-06-13. |
| Abstract | |
The hepatocellular carcinoma (HCC) features a remarkable epidemiological burden, ranking as the third most lethal cancer worldwide. As the HCC-related molecular and cellular complexity unfolds as the disease progresses, the use of a myriad of in vitro models available is mandatory in translational preclinical research setups. In this review paper, we will compile cutting-edge information on the in vitro bioassays for HCC research, (A) emphasizing their morphological and molecular parallels with human HCC; (B) delineating the advantages and limitations of their application; and (C) offering perspectives on their prospective applications. While bidimensional (2D) (co) culture setups provide a rapid low-cost strategy for metabolism and drug screening investigations, tridimensional (3D) (co) culture bioassays - including patient-derived protocols as organoids and precision cut slices - surpass some of the 2D strategies limitations, mimicking the complex microarchitecture and cellular and non-cellular microenvironment observed in human HCC. 3D models have become invaluable tools to unveil HCC pathophysiology and targeted therapy. In both setups, the recapitulation of HCC in different etiologies/backgrounds (i.e., viral, fibrosis, and fatty liver) may be considered as a fundamental guide for obtaining translational findings. Therefore, a "multimodel" approach - encompassing the advantages of different in vitro bioassays - is encouraged to circumvent "model-biased" outcomes in preclinical HCC research. (AU) | |
| FAPESP's process: | 22/13402-9 - Effects of carboxymethylcellulose and polysorbate 80 food additive on hepatocarcinogenesis-associated non-alcoholic fatty liver disease. |
| Grantee: | Gabriel Bacil Prata |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 23/05411-0 - Effects of the combination of coffee compounds and antitumor agents on hepatocellular carcinoma: in vitro and in vivo investigation |
| Grantee: | Leticia Cardoso Valente |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 23/08751-7 - Multimodel Drug Screening Platform (MDSP): Preclinical and Molecular Insights for Colon and Liver Cancer Management. |
| Grantee: | Guilherme Ribeiro Romualdo |
| Support Opportunities: | Scholarships in Brazil - Generation Project |
| FAPESP's process: | 22/16633-1 - Effects of carboxymethylcellulose and polysorbate 80 food additive on nonalcoholic fatty liver disease-associated hepatocarcinogenesis. |
| Grantee: | Luís Fernando Barbisan |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 23/17585-3 - Does Caffeic Acid Enhance the Antitumoral Response of Sorafenib on Hepatocellular Carcinoma? An In Vitro Approach. |
| Grantee: | Luana Riechelmann Casarin |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |