Imidazolium-based ionic liquids as additives to preserve green fluorescent protein activity at room-temperature and under stress

Author(s)

Verissimo, Nathalia Vieira ; Saponi, Carolina Falaschi ; Ryan, Timothy M. ; de Souza Oliveira, Ricardo Pinheiro ; Greaves, Tamar L. ; Brandao Pereira, Jorge Fernando

• Document type: Journal article
• Source: BIOPHYSICAL REVIEWS; v. 13, n. 6, p. 1-pg., 2021-12-01.
• FAPESP's process: 16/07529-5 - Development of liquid-liquid purification process and incorporation in nanostructured polymeric matrix of recombinant green fluorescent protein produced by Escherichia coli
• Grantee: Nathalia Vieira Porphirio Veríssimo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/01858-2 - Study of the stabilizing effect of different solvents on the green fluorescent protein structure produced by Escherichia coli
• Grantee: Carolina Falaschi Saponi
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/06576-5 - Molecular interactions and stability of Green Fluorescent Protein (GFP) in ionic liquids and encapsulation of GFP on reverse micelles
• Grantee: Nathalia Vieira Porphirio Veríssimo
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate (Direct)
• FAPESP's process: 14/19793-3 - Optimization and scale-up of novel Ionic-Liquid-based purification processes for recombinant green fluorescent protein produced by Escherichia coli "GFPurIL"
• Grantee: Sandro Roberto Valentini
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/50009-8 - Understanding the molecular interactions between ionic liquids and biopharmaceuticals: the key for a proper design of downstream processing
• Grantee: Jorge Pereira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/14144-8 - Purification and encapsulation of bacteriocins from probiotic bacteria for applications in animal protein production systems
• Grantee: Nathalia Vieira Porphirio Veríssimo
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/16424-7 - Optimization and scale-up of liquid-liquid extraction process with ionic liquids (ILs) as a sustainable tool for the separation of the anti-leukemia biopharmaceutical L-asparaginase (ASPase)
• Grantee: Jorge Pereira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 18/20833-0 - Entendendo as interações moleculares e a estabilidade da proteína verde fluorescente (GFP) em líquidos iônicos sob condições de estresse
• Grantee: Carolina Falaschi Saponi
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 18/25511-1 - Bioprospecting of bacteriocin-producing bacteria: from culture optimization to the application in animal production systems
• Grantee: Ricardo Pinheiro de Souza Oliveira
• Support Opportunities: Research Projects - Thematic Grants