Statherin-Derived Peptide Protects against Intrinsic Erosive Enamel Wear in situ

Author(s)

Taira, Even Akemi ; Ferrari, Carolina Ruis ; Carvalho, Gabriel ; Pela, Vinicius Taioqui ; Ventura, Talita Mendes Oliveira ; Crusca, Edson ; Marchetto, Reinaldo ; Buzalaf, Marilia Afonso Rabelo

• Document type: Journal article
• Source: Caries Research; v. N/A, p. 6-pg., 2024-09-12.

Abstract

Introduction: This in situ study investigated the protective effect of a solution containing statherin-derived peptide (StatpSpS) against enamel intrinsic erosion (ERO). Methods: Fifteen volunteers wore appliances containing 2 bovine specimens. The samples were subjected to ERO with HCl, mimicking dental ERO by intrinsic acid. The volunteers participated in 3 phases (double-blind and crossover): (1) deionized water (negative control); (2) commercial solution containing SnCl2/NaF/AmF (800 ppm Sn+2, 500 ppm F-, pH 4.5) (positive control); (3) solution containing 1.88 x 10(-5)m StatpSpS. Four times a day, the volunteers administered one drop of the solutions (50 mu L, 1 min) on each specimen. After the treatment, erosive challenges were performed extraorally with 0.01 m HCl (pH 2.0, 4 times/day, 1 min, 150 mL). Enamel wear was assessed by profilometry. Data were analyzed by one-way RM-ANOVA/Bonferroni's tests (p < 0.05). Results: In terms of the treatments, both the commercial solution (SnCl2/NaF/AmF) and StatpSpS significantly reduced the wear when compared to the negative control (p < 0.01), without significant differences between them (p > 0.05). Conclusion: The solution containing StatpSpS demonstrated protection against enamel intrinsic erosive wear. This study marks a significant advancement in the prevention of intrinsic erosive wear, utilizing StatpSpS in acquired pellicle engineering procedures. (AU)

• FAPESP's process: 19/24295-6 - Acquired pellicle engineering for dental erosion control: effect of mouthwash containing derived peptide from statherin on the proteomic profile of the acquired enamel pellicle formed in 3 minutes
• Grantee: Carolina Ruis Ferrari
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 18/12041-7 - Safety and efficacy for the prevention of dental erosion of a new cystatin derived from sugarcane (CaneCPI-5): in vitro, in situ and in vivo studies
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/00203-5 - Effect of saliva enrichment with CaneCPI-5, Stn15pSpS or hemoglobin, isolated or combined, associated or not with EGCG, in thickness, viscoelastic properties, interfacial load and morphology of the acquired enamel pellicle
• Grantee: Talita Mendes Oliveira Ventura
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/26070-1 - Modulation of acquired enamel pellicle to control dental mineral loss: unravelling mechanisms to make therapies possible
• Grantee: Marília Afonso Rabelo Buzalaf
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/04857-4 - Acquired pellicle engineering for control of dental erosion: in situ evaluation of protective potential by a new cistatin derived from sugar cane
• Grantee: Vinícius Taioqui Pelá
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/18749-1 - In vitro evaluation of the protective potential against initial dental erosion of the modification of the acquired enamel pellicle with solutions containing statherin or SERINE-PHOSPHORYLATED or not statherin-derived peptides
• Grantee: Gabriel Carvalho
• Support Opportunities: Scholarships in Brazil - Scientific Initiation