Mapping Biochemical Pathways in Maytenus ilicifolia (Celastraceae) through Integrated Proteomics and Histochemistry

Author(s)

Santos, Vania A. F. M. ; Dias, Nathalia B. ; Teixeira, Simone P. ; Palma, Mario S. ; Furlan, Maysa

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. 32, n. 2, p. 12-pg., 2021-02-01.

Abstract

Maytenus ilicifolia (Celastraceae) is a medicinal plant that is native to southern Brazil and is popularly known as "espinheira-santa". From a biosynthesis perspective, this species accumulates quinonemethide triterpenes and sesquiterpene pyridine alkaloids as major secondary metabolites that exhibit interesting biological properties, with antitumoral and antiprotozoal activities, respectively, being the most frequently reported. Additionally, the restricted accumulation of such compounds in the roots raises questions about the expression of proteins involved in such compartmentalization and their possible biological and/or ecological role in M. ilicifolia. Thus, this article describes the use of shotgun proteomics and histochemical studies for the characterization of the main biosynthetic pathways involved in the regulation of the metabolism in M. ilicifolia roots. This combined approaches also resulted in the identification of a series of proteins involved in the quinonemethide triterpenes and sesquiterpene pyridine alkaloids, providing evidences of their differential compartmentalization. (AU)

• FAPESP's process: 14/50926-0 - INCT 2014: biodiversity and natural products
• Grantee: Vanderlan da Silva Bolzani
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
• Grantee: Glaucius Oliva
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC