Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Determination of parabens in shampoo using high performance liquid chromatography with amperometric detection on a boron-doped diamond electrode

Full text
Author(s):
Martins, Isarita [1] ; Carreira, Franciely Cristiani [2] ; Canaes, Larissa S. [2] ; de Souza Campos Junior, Francisco Alberto [2] ; da Silva Cruz, Leticia Maria [2] ; Rath, Susanne [2]
Total Authors: 6
Affiliation:
[1] Univ Fed Alfenas, Dept Clin & Toxicol Anal, Lab Toxicol Anal, BR-37130000 Alfenas, MG - Brazil
[2] Univ Estadual Campinas, Dept Analyt Chem, Inst Chem, BR-13084971 Campinas, SP - Brazil
Total Affiliations: 2
Document type: Review article
Source: Talanta; v. 85, n. 1, p. 1-7, JUL 15 2011.
Web of Science Citations: 55
Abstract

Methylparaben (MePa), ethylparaben (EtPa) and propylparaben (PrPa) have been widely used, among others, as chemical preservatives in cosmetics, drugs and foods. As these compounds are linked with allergies, dermatitis and estrogenic properties, it is necessary to control the concentration of these substances in different matrices. The aim of this paper are: to evaluate the electrochemical behavior of parabens on the boron-doped diamond (BDD) electrode and the development of a chromatographic method, with electrochemical detection (HPLC-ED), for determination of parabens in shampoo. A BDD (8000 ppm) electrode was adapted in a thin layer mode analytical cell consisting of a stainless steel and a platinum wire as reference and auxiliary electrodes, respectively. Chromatographic separations were obtained with a reversed phase C8 analytical column and a mobile phase of 0.025 mol L(-1) disodium phosphate, pH 7.0, and acetonitrile (40:60, v/v), delivered at a flow rate of 1.0 mL min(-1). Sample preparation was performed by solid phase extraction using C18 cartridges and acetonitrile for elution. Benzylparaben was employed as internal standard. The HPLC-ED method developed, using the BDD electrode, was validated for the determination of parabens in shampoos and presented adequate linearity (>0.999), in the range of 0.0125-0.500% (w/w), detectability 0.01% (w/w), precision (RSD of 2.3-9.8%) and accuracy (93.1-104.4%) and could be applied for routine quality control of shampoos containing MePa. EtPa and PrPa. (C) 2011 Elsevier B.V. All rights reserved. (AU)