Caffeine quantification in dietary supplements using high-throughput on-line solid phase extraction coupled to Venturi easy ambient sonic-spray ionization mass spectrometry

Author(s)

Tega, David Ulisses ; Oliveira, Luan Felipe Campos ; Ferreira, Patrick Cesar ; Soldera, Bruna Beatriz ; Nascimento, Heliara Dalva Lopes ; Eberlin, Marcos Nogueira ; Sussulini, Alessandra

• Document type: Journal article
• Source: ANALYTICAL METHODS; v. 16, n. 17, p. 6-pg., 2024-04-03.

Abstract

Caffeine is present in a large number of beverages and is an additive used in dietary supplements. Therefore, the concern about its quality and safety for consumers has been increasing and hence requires faster and simpler analytical methods to determine the caffeine amount. The high-throughput analysis is an appropriate solution to pharmaceuticals, bioanalysis, forensic and food laboratory routines. In this sense, Venturi easy ambient sonic-spray ionization mass spectrometry (V-EASI-MS), a specific ambient ionization source, is suitable to enable direct analysis of sample solutions in real time and is appropriate to be coupled to liquid chromatography (LC). The development of an on-line solid phase extraction system coupled to V-EASI-MS optimizes the advantages of LC-MS hyphenation by enhancing the figures of merit of the analytical method according to AOAC guidelines and simultaneously minimizing the runtime analysis to 1.5 min per sample, as well as sample preparation steps and solvent consumption, which is currently a challenge for quantitative applications of ambient ionization MS. Optimization of a LC-V-EASI-MS system. Employment of LC-V-EASI-MS and online SPE to enhance analytical performance for caffeine determination reaching 90 s per sample. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/01800-2 - Exploring the potential of cannabis in the treatment of neurodegenerative diseases
• Grantee: Alessandra Sussulini
• Support Opportunities: Regular Research Grants