| Full text | |
| Author(s): |
Martins, Leticia S.
;
Duarte, Evandro L.
;
Lamy, M. Teresa
;
Rozenfeld, Julio H. K.
Total Authors: 4
|
| Document type: | Journal article |
| Source: | Biophysical Chemistry; v. 300, p. 8-pg., 2023-07-12. |
| Abstract | |
The saturated LPC18:0 and unsaturated LPC18:1 lysophosphatidylcholines have important roles in inflammation and immunity and are interesting targets for immunotherapy. The synthetic cationic lipid DODAB has been successfully employed in delivery systems, and would be a suitable carrier for those lysophosphatidylcholines. Here, assemblies of DODAB and LPC18:0 or LPC18:1 were characterized by Differential Scanning Calorimetry (DSC) and Electron Paramagnetic Resonance (EPR) spectroscopy. LPC18:0 increased the DODAB gel-fluid transition enthalpy and rigidified both phases. In contrast, LPC18:1 caused a decrease in the DODAB gel-fluid transition temperature and cooperativity, associated with two populations with distinct rigidities in the gel phase. In the fluid phase, LPC18:1 increased the surface order but, differently from LPC18:0, did not affect viscosity at the membrane core. The impact of the different acyl chains of LPC18:0 and 18:1 on structure and thermotropic behavior should be considered when developing applications using mixed DODAB membranes. (AU) | |
| FAPESP's process: | 16/19077-1 - Development of novel vaccine adjuvants capable of activating CD1d-restricted T cells |
| Grantee: | Julio Henrique Kravcuks Rozenfeld |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 16/13368-4 - Nanostructured systems: from membrane biomimetic models to carriers of bioactives |
| Grantee: | Karin Do Amaral Riske |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 21/01593-1 - Multiple physical techniques to structurally characterize biological relevant membranes and its interactions |
| Grantee: | Maria Teresa Moura Lamy |
| Support Opportunities: | Regular Research Grants |