MicroRNA expression profiling in the adult offspring of rats with periodontal disease

Author(s):Show less -

Mattera, Maria Sara de Lima Coutinho ; Scaramele, Natalia Francisco ; Lopes, Flavia Lombardi ; Belardi, Bianca Elvira ; Tsosura, Thais Veronica Saori ; Sampaio, Heloisa Macedo ; Chiba, Fernando Yamamoto ; Pereira, Renato Felipe ; dos Santos, Rodrigo Martins ; Ervolino, Edilson ; Baliero, Gabriele Fernandes ; Nobumoto, Ana Carla Thalez Ywabuchi ; Cachoni, Anna Clara ; Chaves-Neto, Antonio Hernandes ; Matsushita, Doris Hissako

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 170, p. 9-pg., 2025-02-01.

Abstract

Objective: The present study investigated the relationship between maternal periodontal disease, insulin resistance, activation of inflammatory pathways and epigenetic modifications in adult offspring. Design: Therefore, female Wistar rats were divided into control and experimental groups. Seven days after the induction of periodontal disease, female rats from both groups were mated with healthy male rats. After weaning, male offspring were divided into control offspring (CN-o) and periodontal disease offspring (PED-o) groups. Body weight was measured at 0-75 days of age. At day 75, the following were measured in the offspring: insulin resistance by the HOMA-IR index; global miRNAs by microtranscriptome array; validation of the selected miRNAs by quantitative real-time PCR expression; interleukin 1 receptor associated kinase 1 (IRAK1) and tumor necrosis factor receptor-associated factor 6 (TRAF6) content in the gastrocnemius muscle tissue (GSM) by western blotting. Results: Maternal periodontal disease leads to low birth weight (LBW) in the offspring and insulin resistance in adulthood; changes in global miRNA expression (5 miRNAs upregulated and 6 downregulated); and increased protein expression of IRAK1 and TRAF6 in GSM. Conclusions: These findings demonstrate that maternal periodontal disease causes LBW, insulin resistance, activation of inflammatory pathways, and changes in global miRNA expression. (AU)

• FAPESP's process: 23/03786-7 - EVALUATION OF THE GLOBAL EXPRESSION OF MYRNAS IN WHITE ADIPOSE TISSUE IN ADULT RATS, OFFSPRING OF RATS WITH PERIODONTAL DISEASE
• Grantee: Gabriele Fernandes Baliero
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 22/08872-6 - ANALYSIS OF THE GLOBAL EXPRESSION OF MIRNAS IN GASTROCNEMIUS MUSCLE TISSUE IN ADULT RATS, OFFSPRING OF PERIODONTAL DISEASE
• Grantee: Heloisa Macedo Sampaio
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 23/01400-4 - Evaluation of the melanin effects on insulin signaling pathway and inflammatory in rats with Apical Periodontitis submitted to passive inhalation of tobacco
• Grantee: Doris Hissako Matsushita
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/12488-0 - Evaluation of the melanin effects on insulin signaling pathway and inflammatory in rats with apical periodontitis submitted to passive inhalation of tobacco
• Grantee: Maria Sara de Lima Coutinho Mattera
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• FAPESP's process: 19/04183-9 - STUDY OF MECHANISMS INVOLVED IN THE DEVELOPMENT OF INSULIN RESISTANCE IN ADULT RATS, OFFSPRING OF PERIODONTAL DISEASE
• Grantee: Maria Sara de Lima Coutinho Mattera
• Support Opportunities: Scholarships in Brazil - Post-Doctoral