Developing a nanomaterial-based 3D-printed platform: Application as a cancer aptasensor via detection of heat shock protein 90 (HSP90)

Author(s)

Negahdary, Masoud ; do Lago, Claudimir Lucio ; Gutz, Ivano Gebhardt Rolf ; Buoro, Rafael Martos ; Durazzo, Michelangelo ; Angnes, L. ucio

• Document type: Journal article
• Source: SENSORS AND ACTUATORS B-CHEMICAL; v. 409, p. 10-pg., 2024-03-06.

Abstract

Overexpressed heat shock protein 90 (HSP90) has been proven as a new cancer biomarker, and its detection can be used as an auxiliary diagnosis. In this research, we designed an electrochemical aptasensor to detect this biomarker. Initially, a 3D -printed electrode was designed and produced while the electrode surface (made from a conductive carbon black/ polylactic acid (CB-PLA) filament) was improved by CO2 laser ablation. Then, the electrode surface was modified with graphene oxide (GO) and an array of frost -like gold nanocrystals (FGNCs); the results showed that the final surface covered with gold contained nano/microstructures with a mean width of - 65 nm and a mean length of - 509 nm. Also, a significant increase in the electrochemically active surface area was observed for modified surfaces with GO and FGNCs. Efficient immobilization of aptamer strands on the gold surface could complete the assembly of this aptasensing platform, which could detect analyte molecules in a linear range from 0.1 to 200 ng mL-1. The estimated limit of detection (LOD) and the limit of quantification (LOQ) were as low as 0.1 ng mL-1 and 0.23 ng mL-1, respectively. The performance of reproducibility (5 times), regeneration (5 times), and stability (7 days) were satisfactory (low relative standard deviation (RSD)). Also, this aptasensor showed adequate performance for selective and accurate analyte capturing in the presence of interfering agents and spiked analyte concentrations in serum samples. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/08782-1 - Miniaturized and integrated chemical sensors: new fabrication platforms for biological, clinical and environmental applications
• Grantee: Mauro Bertotti
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 17/13137-5 - Formation and properties of hemiesters of carbonic acid in aqueous medium
• Grantee: Claudimir Lucio Do Lago
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/27021-4 - Design and fabrication of electrochemical biosensors for early diagnosis of myocardial infarction and Hepatocellular Carcinoma using microRNAs (miRNAs) primers as biorecognition element and arrays of gold nanostructures
• Grantee: Masoud Negahdary
• Support Opportunities: Scholarships in Brazil - Post-Doctoral