Effect of Tannin Inclusion on the Enhancement of Rumen Undegradable Protein of Different Protein Sources

Author(s)

Loregian, Kalista E. ; Pereira, David A. B. ; Rigon, Fernanda ; Magnani, Elaine ; Marcondes, Marcos I. ; Baumel, Eduardo A. ; Branco, Renata H. ; Benedeti, Pedro Del Bianco ; Paula, Eduardo M.

• Document type: Journal article
• Source: RUMINANTS; v. 3, n. 4, p. 12-pg., 2023-12-01.

Abstract

Tannins can be utilized to increase rumen undegradable protein (RUP) by their capacity to form complexes with diverse nutrients present in the feed. In that regard, high-performance ruminants demand elevated RUP levels. The objective of this study was to evaluate the effects of incorporating varying levels of tannin into three protein sources (cottonseed, peanut, and soybean meals) on ruminal kinetic parameters, ruminal fermentation, and intestinal digestibility. Thus, three in situ experiments were conducted to investigate the ruminal degradation kinetics, where Fraction A represents the soluble portion, Fraction B relates to the portion potentially degraded in the rumen, and kd denotes the degradation rate of Fraction B, for both dry matter (DM) and crude protein (CP) in the rumen. Additionally, the study assessed dry matter effective degradability (ED), rumen undegradable protein (RUP), and intestinal digestibility (ID). These experiments utilized three cannulated animals for the in situ incubations. Regarding cottonseed meal in terms of DM degradation kinetics, tannin inclusion had a quadratic effect on fraction A (p < 0.01), B (p = 0.10, trend), kd (p = 0.03), and ED (p < 0.01). Fraction A of CP had a cubic effect (p = 0.03), being greater for the control compared with the other treatments. The inclusion of tannin linearly increased RUP (p < 0.01). The RUP proportion increased 29, 33, and 45% when 20, 40, and 60 g/kg tannin were used, respectively, compared to the control. For peanut meal, the A fraction of protein and RUP responded quadratically as tannin was included in peanut meal (p < 0.01). However, tannin levels did not affect fraction B of protein and ID. Regarding soybean meal, fractions A and B of DM and ED had cubic effects (p < 0.01), being greater for the control compared with the other treatments, and responded quadratically as tannin increased. Also, tannin inclusion had a cubic effect on fractions A and B of protein, RUP, and ID (p < 0.01). The cubic behavior showed greater B fraction and ID and lower A fraction and RUP for the control compared other treatments (p < 0.01). Tannins offer a promising avenue for elevating RUP levels in diets featuring cottonseed and peanut meals. Nevertheless, no advantages were observed when treating soybean meal with tannin. (AU)

• FAPESP's process: 17/50339-5 - Institutional research development plan of the Animal Science Institute (PDIp)
• Grantee: Lenira El Faro Zadra
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 18/19743-7 - Effects of different levels of ruminal undegraded protein in beef cattle diets / establishment of in vitro systems (dual-flow continuous culture and gas production) for ruminants' nutrition studies
• Grantee: Eduardo Marostegan de Paula
• Support Opportunities: Research Grants - Young Investigators Grants