Clean modification of potato starch to improve 3D printing of potential bone bio-scaffolds

Author(s)

Sponchiado, Pedro Augusto Invernizzi ; de Melo, Maryanne Trafani ; Bitencourt, Bruna Sousa ; Guedes, Jaqueline Souza ; Tapia-Blacido, Delia Rita ; Augusto, Pedro Esteves Duarte ; Ramos, Ana Paula ; Maniglia, Bianca Chieregato

• Document type: Journal article
• Source: EMERGENT MATERIALS; v. N/A, p. 14-pg., 2024-03-15.

Abstract

Although the 3D printing of biomaterials has witnessed remarkable advancements, there is still a need to develop appropriate bio-based inks. Starches are interesting alternatives to produce hydrogels to be used as inks, but native starches rarely present good performance in 3D printing. To bridge this gap, our study innovatively explores new avenues for the utilization of natural biopolymers in biomedical applications. In this sense, we applied the dry heating treatment (DHT) as a "clean" method to modify potato starch for use in the 3D printing of bone bio-scaffolds. Firstly, the effect of the DHT (1, 2, and 4 h at 130 degrees C) process was evaluated on the structure and physicochemical properties of starch and their hydrogel. Then, 3D-printed bio-scaffolds were obtained from the hydrogels and evaluated in relation to their mechanical performance. DHT promoted starch molecule oxidation and partial depolymerization. The molecular changes resulted in new properties for the obtained hydrogels, such as firmness, cohesion energy, storage modulus (G '), and improved 3D printability-with better accuracy and geometry fidelity. The 3D-printed bio-scaffolds based on DHT starches showed a reduction in the biodegradability rate in relation to the native starch, reducing the swelling power, and improving the mechanical performance-in special DHT 130 degrees C for 1 h. In conclusion, the DHT process is a "clean" and effective method to modify potato starch not only to enhance the performance of the hydrogels but also to improve the properties of the printed biomaterials. (AU)

• FAPESP's process: 21/05947-2 - Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+
• Grantee: Bianca Chieregato Maniglia
• Support Opportunities: Scholarships in Brazil - Young Researchers
• FAPESP's process: 21/06398-2 - Developing ingredients for 3D printing: improving the performance of starch and producing food products
• Grantee: Jaqueline Souza Guedes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/08727-0 - Starch modification by green methods for elaboration via 3D printing of bone scaffolds activated by the presence of hydroxyapatite nanoparticles replaced by Sr2+
• Grantee: Bianca Chieregato Maniglia
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 19/25054-2 - Strontium-containing nanoparticles and their versatility for biomaterials fabrication: implications and applications in biomineralization
• Grantee: Ana Paula Ramos
• Support Opportunities: Regular Research Grants