Insights into PUFA and oxylipin production from incubation of Aspergillus novoparasiticus in sugarcane juice

Author(s)

dos Santos, Augusto Leonardo ; Ferreira, Marcelo Jose Pena ; Sartorelli, Patricia ; da Silva, Josue Jose ; Iamanaka, Beatriz Thie ; Frisvad, Jens Christian ; Taniwaki, Marta Hiromi

• Document type: Journal article
• Source: Food Chemistry; v. 473, p. 10-pg., 2025-02-01.

Abstract

Understanding the metabolism of toxigenic fungi in food is crucial for public health. Fungi of the Aspergillus section Flavi are widespread in tropical and subtropical regions, infecting and producing mycotoxins in peanuts, nuts, cereals, and fruits. Classical Molecular Networking on the Global Natural Products Social Molecular Networking (GNPS) platform was applied to organize and guide the analysis of polyunsaturated fatty acids (PUFAs) and oxylipins, which are strongly associated with chemical communication between fungi and host plants during infection. Dereplication and molecular networking enabled visualization of the metabolome of A. novoparasiticus (ITAL-Y174 strain) incubated in sugarcane juice to mimic the fungal chemical response to plant lipids. The ITAL-Y174 strain produced PUFAs that differed from those of sugarcane. Oxylipins were detected early in the incubation (PGE1, 9,10-DiHOME, 13S-HODE). No known mycotoxins for A. novoparasiticus were detected, and kojic acid was identified, indicating a novel pathway to produce this important industrial natural product. (AU)

• FAPESP's process: 17/50349-0 - Plan of institutional development in research of the Institute of Food Technology - ITAL (PDIp)
• Grantee: Claire Isabel Grígoli de Luca Sarantópoulos
• Support Opportunities: Research Grants - State Research Institutes Modernization Program
• FAPESP's process: 22/09202-4 - Metabolomic and genomic study of fungi aiming at obtaining bioactive substances
• Grantee: Patricia Sartorelli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/00956-3 - Chemoprospection of species of Aspergillus section Flavi isolated from peanuts, Brazil nuts and sugar cane
• Grantee: Augusto Leonardo dos Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/24985-4 - Study the chemical composition of fungi and plants of the Atlantic Forest aimed at antitumor, antiparasitic and antimicrobial potential
• Grantee: Patricia Sartorelli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/12590-3 - Microbial biobanks: promoting innovations for scientific research and technological development (MicroBioBank)
• Grantee: Lara Durães Sette
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/16554-9 - Asteraceae and endophytic fungi: identification of natural products and biological activities
• Grantee: Marcelo José Pena Ferreira
• Support Opportunities: Regular Research Grants