Electrochemical sensor based on nanostructured surface of reduced graphene oxide and gold nanoparticles containing electropolymerized poly-pyrrole for selective determination of limonene enantiomers in orange and pine essential oils

Author(s)

Olsen, Bruna Gabrielle ; Stradiotto, Nelson Ramos

• Document type: Journal article
• Source: Microchemical Journal; v. 209, p. 12-pg., 2025-01-18.

Abstract

Limonene is a compound that has two isomers, (D)-limonene and (L)-limonene, found in different quantities and proportions in citrus fruits and pine tree species. In this work, the construction of the first electrochemical sensor based on nanostructured surface of reduced graphene oxide and gold nanoparticles containing electropolymerized poly-pyrrole for the enantioselective determination of limonene is reported. An analytical curve was obtained, with a limit of detection for (D)-limonene of 1.8 x 10- 12 mol L- 1, limit of quantification of 5.5 x 10-12 mol L-1, and amperometric sensitivity of 5.8 x 105 A L mol-1. For the (L) enantiomer, the corresponding values were 1.4 x 10-12 mol L-1, 4.3 x 10-12 mol L-1, and 6.2 A L mol-1, respectively. The developed sensor was applied for the determination of the two enantiomers in essential oils, where the concentration of (D) and (L)-limonene in orange oil was 8.7 x 10- 2 +/- 6.0 x 10-3 mol L- 1 and 1.1 x 10-3 +/- 2.0 x 10-4 mol L-1, respectively. For the concentration of limonene enantiomers in pine oil, the concentration found for (D) was 1.0 x 10-4 +/- 1.5 x 10-5 mol L- 1 and for (L) was 3.7 x 10-3 +/- 3.3 x 10- 4 mol L- 1, demonstrating the different proportions of each enantiomer. (AU)

• FAPESP's process: 17/22401-8 - Fruit-refinery: obtention processes, characterization methods and generation of products derived from residues of the fruitculture
• Grantee: Nelson Ramos Stradiotto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/00817-9 - Electrochemical sensor based on molecularly imprinted polymer on surface containing metallic nanoparticle and reduced graphene oxide for the enantioselective determination of (D) and (L)-limonene in agroindustrial waste
• Grantee: Bruna Gabrielle Olsen Pinto
• Support Opportunities: Scholarships in Brazil - Master