Swine clones: potential application for animal production and animal models

Author(s):Show less -

Nesiyama, Thais Naomi Gonsalves ; Sangalli, Juliano Rodrigues ; De Bem, Tiago Henrique Camara ; Recchia, Kaiana ; Martins, Simone Maria Massami Kitamura ; de Andrade, Andre Furugen Cesar ; Ferst, Juliana Germano ; Almeida, Gustavo Henrique Dona Rodrigues ; Marques, Mariana Groke ; Doria, Renata Gebara Sampaio ; Carregaro, Adriano Bonfim ; Feliciano, Marcus Antonio Rossi ; Miglino, Maria Angelica ; Fernandes, Fabiana ; Perecin, Felipe ; da Silveira, Juliano Coelho ; Smith, Lawrence Charles ; Bordignon, Vilceu ; Meirelles, Flavio Vieira

• Document type: Journal article
• Source: ANIMAL REPRODUCTION; v. 22, n. 1, p. 13-pg., 2025-01-01.

Abstract

Somatic cell nuclear transfer (SCNT), or cloning, is used to reprogram cells and generate genetically identical embryos and animals. However, the cloning process is inefficient, limiting its application to producing valuable animals. In swine, cloning is mainly utilized to produce genetically modified animals. Indeed, recombinant DNA technologies have evolved considerably in recent years, with homologous recombination and gene editing technologies becoming more efficient and capable of recombining both alleles in a single cell. The selection of appropriate cells and their use as nuclear donors for SCNT is the most common method for generating edited and genetically modified animals for commercial and research purposes. This article reviews current applications of swine cloning and shares our personal experiences with the procedure in this species. (AU)

• FAPESP's process: 21/09886-8 - Epigenetic maturation of the oocyte and totipotence: the potential of the oocyte and its relationship with the ovarian follicular niche
• Grantee: Lawrence Charles Smith
• Support Opportunities: Research Projects - SPEC Program
• FAPESP's process: 23/12977-0 - Manipulation of the Wnt/b-catenin pathway during pig´s oocytes IVM aiming to produce clones by SCNT
• Grantee: Thais Naomi Goncalves Nesiyama
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
• Grantee: Dimas Tadeu Covas
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC