Mapping Thrombosis Serum Markers by ¹H-NMR Allied with Machine Learning Tools

Author(s):Show less -

Martins, Lucas G. ; Manzini, Bruna M. ; Montalvao, Silmara ; Honorato, Millene A. ; Colella, Marina P. ; Hayakawa, Gabriela G. Y. ; de Paula, Erich V. ; Orsi, Fernanda A. ; Braga, Erik S. ; Avramovic, Natasa ; Omage, Folurunsho Bright ; Tasic, Ljubica ; Annichino-Bizzacchi, Joyce M.

• Document type: Journal article
• Source: Molecules; v. 29, n. 24, p. 15-pg., 2024-12-01.

Abstract

Machine learning and artificial intelligence tools were used to investigate the discriminatory potential of blood serum metabolites for thromboembolism and antiphospholipid syndrome (APS). 1H-NMR-based metabonomics data of the serum samples of patients with arterial or venous thromboembolism (VTE) without APS (n = 32), thrombotic primary APS patients (APS, n = 32), and healthy controls (HCs) (n = 32) were investigated. Unique metabolic profiles between VTE and HCs, APS and HCs, and between VTE and triple-positive APS groups were indicative of the significant alterations in the metabolic pathways of glycolysis, the TCA cycle, lipid metabolism, and branched-chain amino acid (BCAA) metabolism, and pointed to the complex pathogenesis mechanisms of APS and VTE. Histidine, 3-hydroxybutyrate, and threonine were shown to be the top three metabolites with the most substantial impact on model predictions, suggesting that these metabolites play a pivotal role in distinguishing among APS, VTE, and HCs. These metabolites might be potential biomarkers to differentiate APS and VTE patients. (AU)

• FAPESP's process: 21/07212-0 - Metabolomic and metabonomic analyzes by nuclear magnetic resonance techniques applied in studies of Venous Thrombosis
• Grantee: Bruna Maria Manzini
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 16/14172-6 - Investigation of the pathophysiological aspects and novel therapeutic approaches for thromboembolic disorders
• Grantee: Joyce Maria Annichino-Bizzacchi
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/02691-2 - Protein exosites, cryptic sites and moonlighting: identification, functional mapping and structural alteration effects
• Grantee: Folorunsho Bright Omage
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/24069-3 - ReSEARCH: Recognizing Signatures of the Exposome to Anticipate the Risks for a Continuous Health
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Research Projects - Thematic Grants