| Full text | |
| Author(s): |
Zamora, Miluska Beatriz Navarrete
;
Acuna, Francisco
;
da Silva, Monica Duarte
;
Santos-Silva, Thamires
;
Garcia, Matheus Henrique Herminio
;
Barreto, Rodrigo da Silva Nunes
;
Sato, Alberto Sato
;
Barbeito, Claudio Gustavo
;
Miglino, Maria Angelica
Total Authors: 9
|
| Document type: | Journal article |
| Source: | BIOLOGY-BASEL; v. 14, n. 1, p. 12-pg., 2025-01-01. |
| Abstract | |
South American camelids inhabit high-altitude environments characterized by hypoxia, influencing embryonic, fetal, and placental development. This study examined the term placenta morphology of alpacas (Vicugna pacos, N = 12) and the immunoexpression of antioxidant selenoproteins (SP). We hypothesize that the placenta of alpacas, adapted to high altitudes, has characteristics with other species also adapted to altitude. Placentas were paraffin-embedded, sectioned (3-5 mu m), stained with hematoxylin-eosin (H&E), Masson's trichrome, and picrosirius red, and analyzed via light and polarized light microscopy. The chorion showed simple cuboidal epithelium with binucleated cells, a subepithelial mesenchyme rich in blood capillaries (area: 124.90 +/- 9.82 mu m2), and type III collagen fibers. The chorionic villi measured 2740.22 +/- 132.75 mu m. The allantois contained a simple columnar epithelium and mesenchyme with type I collagen fibers. Immunohistochemistry localized SP-N, SP-P, Dio-3, and GPx-3 in the blood capillaries and mesenchymal tissue of the chorion but not in the allantois. These findings were compared to human and sheep placentas from different altitudes due to a lack of camelid data at low levels. The morphological features resembled adaptations to hypoxia observed in other species. This preliminary study suggests a potential role for selenoproteins in hypoxia adaptation, providing a basis for future functional studies. (AU) | |
| FAPESP's process: | 21/05445-7 - Functionalized tissue factory: bioengineering based on ecm interactions with biopolymers and bioprinting |
| Grantee: | Maria Angelica Miglino |
| Support Opportunities: | Research Projects - Thematic Grants |