Exploring high molecular weight components in Tityus serrulatus venom

Author(s)

Alano-da-Silva, Nicoly Malachize ; de Oliveira, Isadora Sousa ; Cardoso, Iara Aime ; Bordon, Karla de Castro Figueiredo ; Arantes, Eliane Candiani

• Document type: Journal article
• Source: Toxicon; v. 255, p. 5-pg., 2025-01-17.

Abstract

Our study identified high-molecular-weight compounds from Tityus serrulatus venom (TsV), and most of them have not yet been well explored. TsV was fractionated using FPLC system with different columns, analyzed by SDS-PAGE, and characterized by MALDI-TOF/TOF. Our study showed that TsV contains several high-molecularweight compounds, including CRISPs, metalloproteinase and hyaluronidase. We show how these molecules can be obtained from TsV, enabling future studies about their molecular structures and biological actions, expanding knowledge about this venom. (AU)

• FAPESP's process: 20/13176-3 - Human monoclonal antibodies (scFv) discovery with cross-reactivity and pH-dependent to metalloproteases from Bothrops spp
• Grantee: Isadora Sousa de Oliveira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/10173-6 - Production, modification and characterization of animal toxins with potential biotechnological application
• Grantee: Eliane Candiani Arantes Braga
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/11547-7 - Characterization of high molecular mass components from Tityus serrulatus venom
• Grantee: Nicoly Malachize Alano da Silva
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 21/11936-3 - Center for Translational Science and Biopharmaceutical Development
• Grantee: Benedito Barraviera
• Support Opportunities: Research Grants - Science Centers for Development
• FAPESP's process: 22/08964-8 - Discovery of human monoclonal antibodies (scFv) with cross-reactivity and pH-dependent targeting metalloprotease from Bothrops spp.
• Grantee: Isadora Sousa de Oliveira
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor