Electrophysiological characterization of Ts19 fragment II, a new toxin purified from Tityus serrulatus venom

• Grant number: 13/21342-7
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• Start date: March 01, 2014
• End date: February 28, 2015
• Field of knowledge: Biological Sciences - Pharmacology - Toxicology
• Principal Investigator: Eliane Candiani Arantes Braga
• Grantee: Felipe Augusto Cerni
• Supervisor: Jan Tytgat
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Institution abroad: University of Leuven, Leuven (KU Leuven), Belgium
• Associated to the scholarship: 12/13590-8 - Isolation, molecular and functional characterization of a new toxin from Tityus serrulatus scorpion venom, BP.DR

Abstract

Tityus serrulatus is considered the most dangerous scorpion in Brazil, being responsible for most severe cases of scorpion envenoming. The venom of T. serrulatus presents in its composition: nucleotide, insoluble mucus, mucopolysaccharides, oligopeptide, low molecular weight molecules, protease inhibitors, histamine releasers, amino acids and other organic compounds. Additionally the venom contains numerous low molecular weight proteins with neurotoxic activity of great importance to understand the functioning of ion channels and neuroexcitable phenomena. Its toxins have been extensively studied and many of them have been isolated and characterized. However, it is estimated by mass spectrometry data (unpublished data of the group) that the number of toxins present in the venom is much greater than those previously characterized. Recently, a study by the group found evidence of a new toxin present in the fraction VIB from Tityus serrulatus venom. Thus, this study aims to isolate and characterize structurally (amino acid sequence) and functionally this new toxin. For the functional characterization of the novel toxin will be performed electrophysiological tests on sodium and/or potassium voltage-dependent channels cloned and expressed in oocytes, immunoassays of cytokine release in vitro and biochemical determinations of glucose, AST, ALT , CK, urea and plasma creatinine in vivo. The study of toxins from scorpion venom has been shown relevant for providing the molecular basis involved in the mechanism of its toxicity and identify molecules with potential use as drugs or as tools for studies of different biological systems. (AU)

Scientific publications

(9)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PUCCA, MANUELA B.; BERTOLINI, THAIS B.; CERNI, FELIPE A.; BORDON, KARLA C. F.; PEIGNEUR, STEVE; TYTGAT, JAN; BONATO, VANIA L.; ARANTES, ELIANE C.. Immunosuppressive evidence of Tityus serrulatus toxins Ts6 and Ts15: insights of a novel K+ channel pattern in T cells. Immunology, v. 147, n. 2, p. 240-250, FEB 2016. (12/12954-6, 13/21342-7, 13/21329-0, 12/13590-8)

PUCCA, MANUELA B.; CERNI, FELIPE A.; PEIGNEUR, STEVE; BORDON, KARLA C. F.; TYTGAT, JAN; ARANTES, ELIANE C.. Revealing the Function and the Structural Model of Ts4: Insights into the ``Non-Toxic{''} Toxin from Tityus serrulatus Venom. TOXINS, v. 7, n. 7, p. 2534-2550, JUL 2015. (13/21342-7, 13/21329-0)

PUCCA, MANUELA BERTO; CERNI, FELIPE AUGUSTO; PINHEIRO-JUNIOR, ERNESTO LOPES; ZOCCAL, KARINA FURLANI; FIGUEIREDO BORDON, KARLA DE CASTRO; AMORIM, FERNANDA GOBBI; PEIGNEUR, STEVE; VRIENS, KIM; THEVISSEN, KARIN; ANGELO CAMMUE, BRUNO PHILIPPE; et al. Non-disulfide-bridged peptides from Tityus serrulatus venom: Evidence for proline-free ACE-inhibitors. Peptides, v. 82, p. 44-51, AUG 2016. (13/21342-7, 14/03332-7, 12/13590-8, 13/06380-0, 14/07125-6, 13/21329-0, 11/12317-3)

PUCCA, MANUELA B.; CERNI, FELIPE A.; PEIGNEUR, STEVE; BORDON, KARLA C. F.; TYTGAT, JAN; ARANTES, ELIANE C.. Revealing the Function and the Structural Model of Ts4: Insights into the "Non-Toxic" Toxin from Tityus serrulatus Venom. TOXINS, v. 7, n. 7, p. 17-pg., 2015-07-01. (13/21329-0, 13/21342-7)

PUCCA, MANUELA BERTO; CERNI, FELIPE AUGUSTO; CORDEIRO, FRANCIELLE ALMEIDA; PEIGNEUR, STEVE; CUNHA, THIAGO MATTAR; TYTGAT, JAN; ARANTES, ELIANE CANDIANI. Ts8 scorpion toxin inhibits the Kv4.2 channel and produces nociception in vivo. Toxicon, v. 119, p. 244-252, SEP 1 2016. (12/12954-6, 13/21342-7, 12/13590-8, 13/21329-0, 13/08216-2)

CERNI, FELIPE AUGUSTO; PUCCA, MANUELA BERTO; ZOCCAL, KARINA FURLANI; FRANTZ, FABIANI GAI; FACCIOLI, LUCIA HELENA; ARANTES, ELIANE CANDIANI. Expanding biological activities of Ts19 Frag-II toxin: Insights into IL-17 production. Toxicon, v. 134, p. 18-25, AUG 2017. (14/07125-6, 13/21329-0, 14/03332-7, 13/21342-7)

PUCCA, MANUELA B.; PEIGNEUR, STEVE; COLOGNA, CAMILA T.; CERNI, FELIPE A.; ZOCCAL, KARINA F.; BORDON, KARLA DE C. F.; FACCIOLI, LUCIA H.; TYTGAT, JAN; ARANTES, ELIANE C.. Electrophysiological characterization of the first Tityus serrulatus alpha-like toxin, Ts5: Evidence of a pro-inflammatory toxin on macrophages. Biochimie, v. 115, p. 8-16, AUG 2015. (13/21342-7, 14/03332-7, 13/21329-0, 09/07169-5, 13/26200-6)

CERNI, FELIPE AUGUSTO; PUCCA, MANUELA BERTO; AMORIM, FERNANDA GOBBI; FIGUEIREDO BORDON, KARLA DE CASTRO; ECHTERBILLE, JULIEN; QUINTON, LOIC; DE PAUW, EDWIN; PEIGNEUR, STEVE; TYTGAT, JAN; ARANTES, ELIANE CANDIANI. Isolation and characterization of Ts19 Fragment II, a new long-chain potassium channel toxin from Tityus serrulatus venom. Peptides, v. 80, p. 9-17, JUN 2016. (13/21342-7, 12/13590-8, 13/21329-0, 13/26083-0, 11/12317-3)

PUCCA, MANUELA B.; BERTOLINI, THAIS B.; CERNI, FELIPE A.; BORDON, KARLA C. F.; PEIGNEUR, STEVE; TYTGAT, JAN; BONATO, VANIA L.; ARANTES, ELIANE C.. Immunosuppressive evidence of Tityus serrulatus toxins Ts6 and Ts15: insights of a novel K+ channel pattern in T cells. IMMUNOLOGY, v. 147, n. 2, p. 11-pg., 2016-02-01. (12/12954-6, 13/21329-0, 13/21342-7, 12/13590-8)