Venom proteomics and Duvernoy's venom gland histology of Pseudoboa neuwiedii (Neuwied's false boa; Dipsadidae, Pseudoboini)

Author(s)

Torres-Bonilla, Kristian A. ; Bayona-Serrano, Juan D. ; Saenz-Suarez, Paula A. ; Andrade-Silva, Debora ; Bernal-Bautista, Manuel H. ; Serrano, Solange M. T. ; Hyslop, Stephen

• Document type: Journal article
• Source: Toxicon; v. 254, p. 10-pg., 2024-12-24.

Abstract

The venom of Colombian specimens of the rear-fanged snake Pseudoboa neuwiedii contains proteolytic and phospholipase A2 (PLA2) activities, but is devoid of esterases. Mass spectrometric analysis of electrophoretic bands indicated that this venom contains C-type lectins (CTL), cysteine-rich secretory proteins (CRiSP), PLA2, snake venom metalloproteinases (SVMP), and snake venom matrix metalloproteinases (svMMP). In this investigation, we extended our characterization of P. neuwiedii by undertaking a shotgun proteomic analysis of the venom and comparing the results with a transcriptomic database for Brazilian P. neuwiedii; proteomic data previously obtained by in-gel digestion of electrophoretic bands coupled with mass spectrometry were also reanalyzed by comparing them with the transcriptomic results. The histology of the Duvernoy's venom gland was also examined. Histological analysis revealed a structural organization similar to that of other colubrids that consisted of a serous venom gland and a mucous supralabial gland. When the shotgun proteomic data were run against a general UniProt database for serpents, only metalloproteinases were identified (99% SVMPs, 1% snake endogenous matrix metalloproteinases-9 or seMMP-9). In contrast, when run against a transcriptomic database derived from the venom gland of Brazilian P. neuwiedii that contains predominantly SVMP, CRiSP, type IIE PLA2 (PLA2-IIE), CTL and seMMP-9, the main components identified were seMMP-9 (49%), SVMP (47%), CRiSP (3%) and minor components that included CTL and PLA2-IIE. These findings confirmed the previously reported general composition of P. neuwiedii venom, with metalloproteinases (SVMP and seMMP-9) being the major components, and refined the identification of certain components, e.g., type IIA PLA2 now identified as PLA2-IIE and the detection of seMMP-9 rather than svMMP. The data also indicate compositional similarity between Brazilian and Colombian P. neuwiedii venoms, and stress the need for specific databases for non-front-fanged colubroid snakes to allow accurate and more comprehensive identification of the venom components of these snakes. (AU)

• FAPESP's process: 16/16935-7 - A post-translational modification perspective of Bothrops venom toxins: site-specific proteomic characterization of N-glycans and Cys residues.
• Grantee: Débora Andrade Silva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/50127-5 - Dimensions US-BIOTA São Paulo: scales of biodiversity: integrated studies of snake venom evolution and function across multiple levels of diversity
• Grantee: Inácio de Loiola Meirelles Junqueira de Azevedo
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 13/07467-1 - CeTICS - Center of Toxins, Immune-Response and Cell Signaling
• Grantee: Hugo Aguirre Armelin
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 17/24498-9 - Venomics analyses of Dipsadinae snakes focusing on adaptations to ophiophagy
• Grantee: Juan David Bayona Serrano
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)