Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica

Author(s)

Cides da Silva, L. C. ^[1] ; Infante, C. M. C. ^[1] ; Lima, A. W. O. ^[1] ; Cosentino, I. C. ^[2] ; Fantini, M. C. A. ^[3] ; Rocha, F. R. P. ^[1] ; Masini, J. C. ^[1] ; Matos, J. R. ^[1]

Affiliation

^[1] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, BR-05599970 Sao Paulo - Brazil

^[2] IPEN, Ctr Ciencia & Tecnol Mat, BR-05422970 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Dept Fis Aplicada, Inst Fis, BR-05315970 Sao Paulo - Brazil

• Document type: Journal article
• Source: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC; v. 70, n. 3-4, p. 149-153, JUL 2011.
• Web of Science Citations: 7

Abstract

Large pore ordered mesoporous silica FDU-1 with three-dimensional (3D) face-centered cubic, Fm3m arrangement of rnesopores, was synthesized under strong acid media using B-50-6600 poly(ethylene oxide)-poly(butylene oxide)-poly(ethylene oxide) triblock copolymer (EO(39)BO(47)EO(39)), tetraethyl orthosilicate (TEOS) and trimethyl-benzene (TMB). Large pore FDU-1 silica was obtained by using the following gel composition 1TEOS:0.00735B50-6600:0.00735TMB:6HCl:155H(2)O. The pristine material exhibited a BET specific surface area of 684 m(2) g(-1), total pore volume of 0.89 cm(3) g(-1), external surface area of 49 m(2) g(-1) and microporous volume of 0.09 cm(3) g(-1). The enzyme activity was determined by the Flow Injection Analysis-Chemiluminescence (FIA-CL) method. For GOD immobilized on the FDU-1 silica, GOD supernatant and GOD solution, the FIA-CL results were 9.0, 18.6 and 34.0 U, respectively. The value obtained for the activity of the GOD solution with FIA-CL method is in agreement with the 35 U, obtained by spectrophotometry. (C) 2011 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 08/09284-3 - Development of methodologies for determination of organic substances using Sequential Injection Analysis (SIA) and high performance liquid chromatography in monolithic columns
• Grantee: Carlos Martín Infante Córdova
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 07/07646-2 - Potential applications of highly ordered mesoporous materials in analytical chemistry and the environment
• Grantee: Luís Carlos Cides da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral