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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Immobilization of glucose oxidase enzyme (GOD) in large pore ordered mesoporous cage-like FDU-1 silica

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Author(s):
Cides da Silva, L. C. [1] ; Infante, C. M. C. [1] ; Lima, A. W. O. [1] ; Cosentino, I. C. [2] ; Fantini, M. C. A. [3] ; Rocha, F. R. P. [1] ; Masini, J. C. [1] ; Matos, J. R. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Dept Quim Fundamental, Inst Quim, BR-05599970 Sao Paulo - Brazil
[2] IPEN, Ctr Ciencia & Tecnol Mat, BR-05422970 Sao Paulo - Brazil
[3] Univ Sao Paulo, Dept Fis Aplicada, Inst Fis, BR-05315970 Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC; v. 70, n. 3-4, p. 149-153, JUL 2011.
Web of Science Citations: 7
Abstract

Large pore ordered mesoporous silica FDU-1 with three-dimensional (3D) face-centered cubic, Fm3m arrangement of rnesopores, was synthesized under strong acid media using B-50-6600 poly(ethylene oxide)-poly(butylene oxide)-poly(ethylene oxide) triblock copolymer (EO(39)BO(47)EO(39)), tetraethyl orthosilicate (TEOS) and trimethyl-benzene (TMB). Large pore FDU-1 silica was obtained by using the following gel composition 1TEOS:0.00735B50-6600:0.00735TMB:6HCl:155H(2)O. The pristine material exhibited a BET specific surface area of 684 m(2) g(-1), total pore volume of 0.89 cm(3) g(-1), external surface area of 49 m(2) g(-1) and microporous volume of 0.09 cm(3) g(-1). The enzyme activity was determined by the Flow Injection Analysis-Chemiluminescence (FIA-CL) method. For GOD immobilized on the FDU-1 silica, GOD supernatant and GOD solution, the FIA-CL results were 9.0, 18.6 and 34.0 U, respectively. The value obtained for the activity of the GOD solution with FIA-CL method is in agreement with the 35 U, obtained by spectrophotometry. (C) 2011 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 08/09284-3 - Development of methodologies for determination of organic substances using Sequential Injection Analysis (SIA) and high performance liquid chromatography in monolithic columns
Grantee:Carlos Martín Infante Córdova
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 07/07646-2 - Potential applications of highly ordered mesoporous materials in Analytical Chemistry and the environment
Grantee:Luís Carlos Cides da Silva
Support type: Scholarships in Brazil - Post-Doctorate