Building a digital library on research into mineralizing vesicles: a systematic review-based approach

Author(s)

Chaves Filho, Gildacio Pereira ; Tavares, Pedro de Andrade ; de Jesus, Ananda Fernanda ; Ciancaglini, Pietro ; Segundo, Jose Eduardo Santarem ; Ramos, Ana Paula

• Document type: Journal article
• Source: BIOPHYSICAL REVIEWS; v. N/A, p. 25-pg., 2025-02-13.

Abstract

This systematic review consolidates current research on mineralizing extracellular vesicles, or matrix vesicles (MVs), including their isolation, characterization, and role in physiological and pathological calcification. We searched PubMed/Medline, Scopus, and Web of Knowledge by employing the keywords "matrix vesicles" or "collagenase-released matrix vesicles" or "mineralizing vesicles" and publishing years from 2000 to 2023. Seventy-one studies met the inclusion criteria. The studies described different experimental protocols, especially with respect to methods for isolating MVs, wherein digestion with collagenase combined with centrifugation was the most used. The studies employed characterization techniques, including the determination of alkaline phosphatase (ALP) and transmission electron microscopy (TEM), to assess the functionality, size, and morphology of MVs. MVs contain key proteins such as ALP, annexins, and osteocalcin, along with calcium and phosphate ions, which are all critical for precipitating apatite. In the studies, evaluation of ALP activity revealed that MVs are more effective for mineralization than their parent cells and, hence, a valuable tool to regenerate bone and to engineer tissues. On the other hand, MVs play an essential role in pathologies, and the studies showed how they contribute to vascular calcification. Despite the therapeutic potential of MVs, isolation methods and characterization protocols vary across the studies, so standardized methods are needed. We have consolidated the data resulting from this systematic review in an open digital library on MVs with free access to all researchers. The users of the digital library can apply filters and taxonomy to find and interconnect the data resulting from the review. (AU)

• FAPESP's process: 19/25054-2 - Strontium-containing nanoparticles and their versatility for biomaterials fabrication: implications and applications in biomineralization
• Grantee: Ana Paula Ramos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/08568-2 - Investigation of the extracellular vesicles (VEs) role in the initiation, propagation, regeneration, and modeling of biological mineralization
• Grantee: Pietro Ciancaglini
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/03349-0 - LINKED DATA SOURCES SELECTION FLOW FOR SEMANTIC ENRICHMENT OF SCIENTIFIC COMMUNICATION DATA.
• Grantee: Ananda Fernanda de Jesus
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 23/07244-4 - Development of cells co-culture models to assess the effect of new strontium and flavonoids complexes with application in bone and cartilage regeneration
• Grantee: Gildacio Pereira Chaves Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral