Green Extraction and NMR Analysis of Bioactives from Orange Juice Waste

Author(s):Show less -

Penteado, Paula Scarabotto ; Leal, Maria Carolina B. Di-Medeiros ; Carosio, Maria Gabriela Aparecida ; dos Santos, Alef ; Segatto, Mateus Lodi ; Pavarini, Daniel Petinatti ; da Silva, Danielle Fernandes ; Amaral, Jessica Cristina ; da Silva, Maria Fatima das G. F. ; Zeidler, Vania G. Zuin ; Ferreira, Antonio G.

• Document type: Journal article
• Source: FOODS; v. 14, n. 4, p. 14-pg., 2025-02-01.

Abstract

Brazil is a global leader in the orange industry, producing approximately one-fourth of the world's oranges and generating over 50% of the associated waste. These by-products are rich in bioactive compounds; however, their improper disposal poses environmental risks. This study employs an eco-friendly approach-microwave-assisted extraction-to recover valuable compounds from orange juice production waste. The extracted compounds were analyzed using nuclear magnetic resonance (NMR) and gas chromatography-mass spectrometry (GC-MS). Key bioactives, including D-limonene, valencene, hesperidin, and carbohydrates, were successfully identified. NMR effectively traces and semi-quantifies these compounds, while microwave-assisted extraction enables the sustainable recovery of high-purity hesperidin, confirmed by NMR (87.66%) and HPLC (84.30%) analyses. (AU)

• FAPESP's process: 19/10756-1 - Metabolomic analysis by Nuclear Magnetic Resonance of blood samples from individuals submitted to cardiorespiratory rehabilitation.
• Grantee: Roberta Manzano Maria
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/07806-4 - Understanding and managing resistance, including novel methods, for pathogen and pest control
• Grantee: Daniel Petinatti Pavarini
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/25015-1 - Design and application of green methods for the extraction of high value-added organic compounds from agro-industrial residues
• Grantee: Vânia Gomes Zuin Zeidler
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/50918-7 - INCT 2014: for the Biorational Control of Pest-Insect and Phytopathogens
• Grantee: Maria Fátima das Graças Fernandes da Silva
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/11409-0 - Reuse of agro-industrial fruit residue from conventional and organic agriculture: green and sustainable extraction methods for bioactive compounds
• Grantee: Mateus Lodi Segatto
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)