Ionic Liquids as Green Extractors for Achievement of Carotenoids: Study of Bioavailability and Antioxidant Activit

• Grant number: 16/18910-1
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2017
• End date: January 31, 2019
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Veridiana Vera de Rosso
• Grantee: Veridiana Vera de Rosso
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated researchers: Paula Larangeira Garcia Martins

Abstract

The application of ionic liquids (IL) in extraction processes is a viable alternative, which follows the principles of green chemistry, minimizing contamination and optimizing the achievement of these natural compounds. Thus, the aim of this study will be establish a new method of extraction using IL for achievement of carotenoids from orange peel and pupunha pulp (Bactrys gasipaes), which can be used as natural pigments and additives in the food industry. Four different IL will be tested as extractors, the 1-n-butyl-3-methylimidazolium tetrafluoroborate and the 1-n-hexyl-3-methylimidazolium chloride from trademark; and the 1-n-butyl-3-methylimidazolium chloride and the 1-n-butyl-3-methylimidazolium hexafluorophosphate synthesized in laboratory. The synthesis assay of the IL will be assisted by ultrasound, obtained without the use of harmful organic solvents, and the characterization of the IL will be performed by mass spectrometry (MS). Preliminary tests will be conducted for select the most effective IL for extraction of carotenoids. The efficiency parameter assessed will be the yield in extraction of carotenoids (quantification by external calibration curve in HPLC-PDA). The two best IL will be selected to optimize the extraction using the response surface technique, through a fractional factorial design, followed by a full factorial design. The qualitative and quantitative determination of carotenoids extracts will be performed by HPLC-PAD-MS/MS. The stability of carotenoids extracts obtained by conventional extraction and by IL will be assessed in relation to the presence and absence of light and at different storage temperatures. For carotenoids extracts, stability will be further evaluated in two distinct systems: oily and watery. The bioavailability in cell model system (Caco2) and antioxidant activity will be compared with carotenoid extracts obtained by conventional methods employing acetone as solvent extractor. With these results we intend to advance in the study of the properties of ionic liquids in obtaining bioactive ingredients, enabling the development of processes with less waste, and with the possibility of patent filing. (AU)

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MURADOR, DANIELLA C.; SALAFIA, FABIO; ZOCCALI, MARIOSIMONE; MARTINS, PAULA L. G.; FERREIRA, ANTONIO G.; DUGO, PAOLA; MONDELLO, LUIGI; DE ROSSO, VERIDIANA V.; GIUFFRIDA, DANIELE. Green Extraction Approaches for Carotenoids and Esters: Characterization of Native Composition from Orange Peel. ANTIOXIDANTS, v. 8, n. 12, DEC 2019. (17/20861-1, 15/26789-5, 16/18910-1)

VANNUCHI, NICHOLAS; CAVALCATE BRAGA, ANNA RAFAELA; DE ROSSO, VERIDIANA VERA. High-Performance Extraction Process of Anthocyanins from Jussara (Euterpe edulis) Using Deep Eutectic Solvents. PROCESSES, v. 10, n. 3, p. 18-pg., 2022-03-01. (16/18910-1, 18/04265-2)

VIGANO, JULIANE; DE AGUIAR, ANA CAROLINA; VEGGI, PRISCILLA CARVALHO; SANCHES, VITOR L.; ROSTAGNO, MAURICIO ARIEL; MARTINEZ, JULIAN. Techno-economic evaluation for recovering phenolic compounds from acai (Euterpe oleracea) by-product by pressurized liquid extraction. JOURNAL OF SUPERCRITICAL FLUIDS, v. 179, JAN 2022. (20/07575-2, 17/23670-2, 16/18910-1, 19/13496-0, 20/15774-5)

DE SOUZA MESQUITA, LEONARDO M.; MURADOR, DANIELLA CARISA; NEVES, BRUNA VITORIA; CAVALCANTE BRAGA, ANNA RAFAELA; PISANI, LUCIANA PELLEGRINI; DE ROSSO, VERIDIANA VERA. Bioaccessibility and Cellular Uptake of Carotenoids Extracted from Bactris gasipaes Fruit: Differences between Conventional and Ionic Liquid-Mediated Extraction. Molecules, v. 26, n. 13, JUL 2021. (16/23242-8, 16/18910-1)

DE SOUZA MESQUITA, LEONARDO M.; NEVES, BRUNA VITRIA; PISANI, LUCIANA PELLEGRINI; DE ROSSO, VERIDIANA VERA. Mayonnaise as a model food for improving the bioaccessibility of carotenoids from Bactris gasipaes fruits. LWT-FOOD SCIENCE AND TECHNOLOGY, v. 122, MAR 2020. (16/23242-8, 16/18910-1)

MURADOR, DANIELLA C.; MESQUITA, LEONARDO M. DE SOUZA; NEVES, V, BRUNA; BRAGA, ANNA RC; MARTINS, PAULA L. G.; ZEPKA, LEILA Q.; DE ROSSO, VERIDIANA V.. Bioaccessibility and cellular uptake by Caco-2 cells of carotenoids and chlorophylls from orange peels: A comparison between conventional and ionic liquid mediated extractions. Food Chemistry, v. 339, MAR 1 2021. (15/26789-5, 16/18910-1)

DE SOUZA MESQUITA, LEONARDO M.; MARTINS, MARGARIDA; PISANI, LUCIANA P.; VENTURA, SONIA P. M.; DE ROSSO, VERIDIANA V.. Insights on the use of alternative solvents and technologies to recover bio-based food pigments. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, v. 20, n. 1, DEC 2020. (16/23242-8, 16/18910-1, 18/10705-5)

DE SOUZA MESQUITA, LEONARDO M.; MARTINS, MARGARIDA; MARICATO, ELIA; NUNES, CLAUDIA; QUINTEIRO, PAULA S. G. N.; DIAS, V, ANA C. R.; COUTINHO, JOAO A. P.; PISANI, LUCIANA P.; DE ROSSO, VERIDIANA V.; VENTURA, SONIA P. M.. Ionic Liquid-Mediated Recovery of Carotenoids from the Bactris gasipaes Fruit Waste and Their Application in Food-Packaging Chitosan Films. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 8, n. 10, p. 4085-4095, MAR 16 2020. (18/10705-5, 16/23242-8, 16/18910-1)

GARCIA MARTINS, PAULA LARANGEIRA; BRAGA, ANNA RAFAELA; DE ROSSO, VERIDIANA VERA. Can ionic liquid solvents be applied in the food industry?. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 66, p. 117-124, AUG 2017. (16/18910-1)

DE SOUZA MESQUITA, LEONARDO M.; VENTURA, SONIA P. M.; BRAGA, ANNA R. C.; PISANI, LUCIANA P.; DIAS, V, ANA C. R.; DE ROSSO, VERIDIANA V.. Ionic liquid-high performance extractive approach to recover carotenoids from Bactris gasipaes fruits. GREEN CHEMISTRY, v. 21, n. 9, p. 2380-2391, MAY 7 2019. (16/23242-8, 16/18910-1)

DE SOUZA MESQUITA, LEONARDO M.; MENNITTI, V, LAIS; DE ROSSO, VERIDIANA V.; PISANI, LUCIANA P.. The role of vitamin A and its pro-vitamin carotenoids in fetal and neonatal programming: gaps in knowledge and metabolic pathways. NUTRITION REVIEWS, v. 79, n. 1, p. 76-87, JAN 2021. (19/09359-8, 16/18910-1, 16/23242-8, 16/14133-0)

MURADOR, DANIELLA C.; DE SOUZA MESQUITA, LEONARDO M.; VANNUCHI, NICHOLAS; BRAGA, ANNA RAFAELA C.; DE ROSSO, VERIDIANA V.. Bioavailability and biological effects of bioactive compounds extracted with natural deep eutectic solvents and ionic liquids: advantages over conventional organic solvents. CURRENT OPINION IN FOOD SCIENCE, v. 26, n. SI, p. 25-34, APR 2019. (15/26789-5, 16/18910-1)

DE SOUZA MESQUITA, LEONARDO M.; CASAGRANDE, BRENO P.; SANTAMARINA, ALINE B.; SERTORIO, MARCELA N.; DE SOUZA, DANIEL VITOR; MENNITTI, LAIS V.; JUCA, ANDREA; JAMAR, GIOVANA; ESTADELLA, DEBORA; RIBEIRO, DANIEL ARAKI; et al. Carotenoids obtained from an ionic liquid-mediated process display anti-inflammatory response in the adipose tissue-liver axis. FOOD & FUNCTION, v. 12, n. 18, JUN 2021. (16/23242-8, 16/18910-1)

MURADOR, DANIELLA C.; BRAGA, ANNA RAFAELA C.; MARTINS, PAULA L. G.; MERCADANTE, ADRIANA Z.; DE ROSSO, VERIDIANA V.. Ionic liquid associated with ultrasonic-assisted extraction: A new approach to obtain carotenoids from orange peel. Food Research International, v. 126, DEC 2019. (15/26789-5, 16/18910-1)

MURADOR, DANIELLA C.; DE SOUZA MESQUITA, LEONARDO M.; VANNUCHI, NICHOLAS; BRAGA, ANNA RAFAELA C.; DE ROSSO, VERIDIANA V.. Bioavailability and biological effects of bioactive compounds extracted with natural deep eutectic solvents and ionic liquids: advantages over conventional organic solvents. CURRENT OPINION IN FOOD SCIENCE, v. 26, p. 10-pg., 2019-04-01. (16/18910-1, 15/26789-5)