Microplastic Interference with Fipronil Toxicity to Zebrafish Embryonic Development

Author(s)

Tominaga, Flavio Kiyoshi ; Brito, Rafaella Silva ; dos Santos, Isis Wanessa Ferreira ; Valle, Amanda de Azevedo ; Giannocco, Gisele ; Maciel, Rui Monteiro de Barros ; Kummrow, Fabio ; Souza, Marilia Cristina Oliveira ; Luchiari, Ana Carolina ; Pereira, Bruno Fiorelini

• Document type: Journal article
• Source: MICROPLASTICS; v. 3, n. 4, p. 13-pg., 2024-12-01.

Abstract

Environmental contamination from mixtures of chemical substances and materials resulting from anthropogenic activities has adverse effects on aquatic biota. Fipronil (FP) is an insecticide widely used in agriculture and public health and is frequently detected in environmental compartments. Microplastics (MPs) represent another class of contaminants that have raised concerns due to their ability to adsorb pollutants, potentially acting as a vector. This study evaluated the influence of polyethylene microplastics on the toxicity of FP in zebrafish (Danio rerio) embryos/larvae. Zebrafish embryos were exposed to aqueous suspension containing purified FP and the FP + MP mixtures for 120 h. Mortality, teratogenicity and cardiotoxicity were observed during the zebrafish development. Larvae were more sensitive to FP than the embryo. The presence of MPs reduced lethality; however, no significant changes were observed for sublethal endpoints. Our findings demonstrate the acute impact of FP on zebrafish embryos/larvae, demonstrating that MPs interfere with toxicity. Therefore, understanding the mechanisms of interaction between contaminants of different classes is essential for predicting the associated risks to biota. (AU)

• FAPESP's process: 23/09884-0 - Analysis of histopathological effects and endocrine disruption in chronic exposure to Diflubenzuron and Piriproxyfen in the experimental model Danio rerio
• Grantee: Rafaella da Silva Brito
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 24/01418-3 - JOINT EFFECTS OF MICROPLASTIC AND INSECTICIDES APPLIED IN PUBLIC HEALTH CAMPAIGNS AND VETERINARY MEDICINE ON DIFFERENT STAGES OF DEVELOPMENT SPECIES DANIO RERIO (ZEBRAFISH).
• Grantee: Bruno Fiorelini Pereira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/03094-5 - Project Zebratox: toxicological screening of larvicides of public and veterinary health importance
• Grantee: Bruno Fiorelini Pereira
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 24/10993-1 - Joint effects of microplastic and insecticides applied in public health campaigns and veterinary medicine on different stages of development species danio rerio (zebrafish)
• Grantee: Flavio Kiyoshi Tominaga
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• FAPESP's process: 23/15540-2 - TOXICOLOGICAL EVALUATION OF DIFFERENT FORMULATIONS OF DIFLUBENZURON: ANALYSIS OF MULTIPLE MORPHOLOGICAL BIOMARKERS IN ZEBRAFISH (Danio rerio)
• Grantee: Amanda de Azevedo Valle
• Support Opportunities: Scholarships in Brazil - Master