Synthesis and Characterization of a Strontium-Quercetin Complex and Its In Vitro and In Vivo Potential for Application in Bone Regeneration

Author(s):Show less -

Pimenta, Israel B. ; Chaves Filho, Gildacio ; Silva, Elias G. B. ; Nogueira, Lucas F. B. ; de Mendonca, Tomaz Santana ; Furtado, Taissa C. S. ; Ferreira GasparNeto, Paulo Cesar ; Dias, Luis Gustavo ; Fukada, Sandra Yasuyo ; Ciancaglini, Pietro ; Ramos, Ana Paula

• Document type: Journal article
• Source: ACS OMEGA; v. 10, n. 5, p. 11-pg., 2025-01-30.

Abstract

Progress has been made toward developing therapies to treat bone-related diseases and defects caused by trauma. However, some of these therapies, such as administering strontium ranelate to treat osteoporosis, have significant side effects. In this context, designing new and safer strontium-based materials constitutes an important current challenge. Here, we have used quercetin as a platform to synthesize a new complex based on strontium and evaluate its activity in vitro and in vivo. First, we carried out strontium complexation with quercetin. Then, we employed Fourier transform infrared spectroscopy, nuclear magnetic resonance, and thermal gravimetric analysis to determine the chemical composition of the resulting complex as [(C15H7O7)Sr2]<middle dot>6(H2O), which was also supported by theoretical calculations. This complex enhanced osteogenic differentiation of a preosteoblastic cell line in vitro, which increased alkaline phosphatase activity and extracellular matrix mineralization. By using a periapical lesion model in mice, we tested whether treatment with this complex could regenerate bone defects in vivo and found that the lesions decreased after 7 days. Together, our data showed that the strontium-quercetin complex synthesized herein is a potential candidate for developing new bone regeneration therapies. (AU)

• FAPESP's process: 19/25054-2 - Strontium-containing nanoparticles and their versatility for biomaterials fabrication: implications and applications in biomineralization
• Grantee: Ana Paula Ramos
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/08568-2 - Investigation of the extracellular vesicles (VEs) role in the initiation, propagation, regeneration, and modeling of biological mineralization
• Grantee: Pietro Ciancaglini
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 23/07244-4 - Development of cells co-culture models to assess the effect of new strontium and flavonoids complexes with application in bone and cartilage regeneration
• Grantee: Gildacio Pereira Chaves Filho
• Support Opportunities: Scholarships in Brazil - Post-Doctoral