Can pigments of different natures interfere with the cytotoxicity from in-office bleaching?

Author(s)

Ribeiro, Rafael Antonio de Oliveira ; Martins, Beatriz Voss ; Dias, Marlon Ferreira ; Peruchi, Victoria ; Anselmi, Caroline ; Soares, Igor Paulino Mendes ; Hebling, Josimeri ; Cavalli, Vanessa ; Costa, Carlos Alberto de Souza

• Document type: Journal article
• Source: ODONTOLOGY; v. N/A, p. 9-pg., 2025-02-13.

Abstract

Objective To assess the influence of tooth stained with different colored solutions on the cytotoxicity of a 35% H2O2-bleaching gel. Materials and methods The established groups were (n = 8): NC- without treatment (negative control); PC- 35% H2O2 (positive control); CO- coffee + 35% H2O2; BT- black tea + 35% H2O2; WI- wine + 35% H2O2; COBTWI- coffee/black tea/wine + 35% H2O2. Standardized enamel/dentin disks were submitted to staining protocols, adapted to artificial pulp chambers, and bleaching was performed. The chromatic alterations (Delta E-00,E- Delta WID) were assessed after bleaching and the extracts (culture medium + diffused gel components) were collected and applied to odontoblast-like cells. The amount of diffused H2O2, cell viability, and oxidative stress were evaluated and data were submitted to ANOVA/Tukey; p < 0.05). Results Thirty five% H2O2 effectively bleached the stained specimens, regardless of the staining solution used (CO, BT, WI, and COBTWI), and no differences were found among the stained protocols (p > 0.05). All staining protocols reduced H2O2 trans-amelodentinal diffusion (p > 0.05), decreasing bleaching cytotoxicity in comparison to PC (p < 0.05). Conclusion Irrespective of the nature of the pigment used for staining enamel and dentin, the gel with 35% H2O2 effectively bleached the tooth structure. The presence of pigments in both dental tissues, regardless their nature, reduced the trans-amelodentinal diffusion of H2O2 and consequently the cytotoxicity of the bleaching gel. (AU)

• FAPESP's process: 23/02780-5 - Development and application of a TiF4-loaded polymeric primer for in-office dental bleaching associated with violet LED irradiation
• Grantee: Carlos Alberto de Souza Costa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 21/01184-4 - Development of an innovative strategy for in-office tooth bleaching: aesthetic effectivity and biocompatibility associated with reduced clinical time
• Grantee: Rafael Antonio de Oliveira Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate