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Biopolymer from the Puree of Mesocarp of Citrullus lanatus (Watermelon) Containing Chlorhexidine Digluconate: Physical-Chemistry Characterization and Biological Aspects

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Paris Junior, Jose Alberto ; Nogueira, Leonardo Gorla ; Cerqueira, Igor Henrique ; Catirse, Nicole Pichirilli ; da Silva, Lucas Henrique Domingos ; Fernandes, Paula de Abreu ; Colturato, Vitoria Maria Medalha ; Dias, Diogenes dos Santos ; Ribeiro, Clovis Augusto ; Dametto, Alessandra Cristina ; Silva, Jhonatan Miguel ; Barud, Hernane da Silva ; Resende, Flavia Aparecida
Total Authors: 13
Document type: Journal article
Source: WASTE AND BIOMASS VALORIZATION; v. N/A, p. 15-pg., 2024-10-10.
Abstract

Fruit and vegetable waste is a promising source to produce functional polymeric films, given the urgent need to replace petroleum-based polymeric materials due to their environmental impacts and limited sustainability. In the present study, we produced an antimicrobial surface film derived from watermelon mesocarp containing chlorhexidine in digluconate form (W-CHX) with applicability for coating 2D surfaces and medical devices. The films were obtained by hydrothermal steps and casting, and characterized by scanning electron microscopy, Fourier transform infrared spectroscopy, thermal stability profile, water barrier properties, fluid handling ability, and drug release, in addition to the assessment of antibacterial, cytotoxic, and mutagenic potential. Although the W-CHC films have demonstrated promising mechanical and antibacterial properties against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, their cytotoxicity and genotoxicity in keratinocytes (HaCaT cells) as revealed by the Comet assay highlight the need for cautious use for topical applications. Therefore, the attributes exhibited by W-CHX films offer a pathway to address the escalating need for biopolymer-based materials with enhanced performance, particularly in extracorporeal applications necessitating microbial growth inhibition. Graphical AbstractAntimicrobial surface film: watermelon mesocarp-only biopolymers with chlorhexidine (CHX) (AU)

FAPESP's process: 19/25318-0 - Multi-User Equipment approved in grant 2017/18782-6: Microscope Leica DM4
Grantee:Eliane Trovatti
Support Opportunities: Multi-user Equipment Program
FAPESP's process: 17/16278-9 - Study of the genotoxicological potential and permeation assay with Caco-2 cells of metallic complexes with promising biological activities
Grantee:Flávia Aparecida Resende Nogueira
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
Grantee:Vanderlei Salvador Bagnato
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC