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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

No effect of low-level lasers on in vitro myoblast culture

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Author(s):
Mesquita-Ferrari, Raquel A. [1] ; Ribeiro, Rafael [1] ; Souza, Nadhia H. C. [1] ; Silva, Camila A. A. [1] ; Martins, Manoela D. [1] ; Bussadori, Sandra K. [1] ; Fernandes, Kristianne P. S. [1]
Total Authors: 7
Affiliation:
[1] Nove de Julho Univ, Dept Rehabil Sci, Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY; v. 49, n. 6, p. 423-428, JUN 2011.
Web of Science Citations: 9
Abstract

Effects of phototherapy using low-level lasers depend on irradiation parameters and the type of laser used. The aim of the present study was to evaluate the effect of phototherapy on the proliferation of cultured C2C12 myoblasts under different nutritional conditions using low-level GaAlAs and InGaAlP lasers with different parameters and incubation periods. C2C12 cells cultured in regular and nutrient-deficient medium were irradiated with low-level GaAlAs (780 nm) and InGaAlP (660 nm) lasers with energy densities of 3.8, 6.3 and 10 J/cm(2), and 3.8, 10 and 17.5 J/cm(2), respectively. Cell proliferation was assessed 48 and 72 h after irradiation by MTT assay. There were no significant differences in cell proliferation between laser-treated myoblasts and control cultures for any of the parameters and incubation periods. Further studies are necessary to determine the correct laser parameters for optimizing the biostimulation of myoblasts. (AU)

FAPESP's process: 10/06116-2 - Effect of anabolic steroid nandrolone decanoate on the proliferation and differentiation of osteoblasts
Grantee:Nadhia Helena Costa Souza
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/09191-5 - Evaluation of the effect of low level laser therapy in the proliferation and differentiation of myoblasts C2C12
Grantee:Raquel Agnelli Mesquita Ferrari
Support Opportunities: Regular Research Grants