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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats

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Author(s):
Soci, U. P. R. [1] ; Fernandes, T. [1] ; Hashimoto, N. Y. [1] ; Mota, G. F. [1] ; Amadeu, M. A. [1] ; Rosa, K. T. [2] ; Irigoyen, M. C. [2] ; Phillips, M. I. [3] ; Oliveira, E. M. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Sch Phys Educ & Sport, Lab Biochem Motor Act, BR-05508900 Sao Paulo - Brazil
[2] Univ Sao Paulo, Hypertens Unit, Heart Inst InCor, Sch Med, BR-05508900 Sao Paulo - Brazil
[3] Keck Grad Inst Appl Life Sci, Lab Stem Cells, Claremont, CA - USA
Total Affiliations: 3
Document type: Journal article
Source: Physiological Genomics; v. 43, n. 11, p. 665-673, JUN 2011.
Web of Science Citations: 88
Abstract

Soci UPR, Fernandes T, Hashimoto NY, Mota GF, Amadeu MA, Rosa KT, Irigoyen MC, Phillips MI, Oliveira EM. MicroRNAs 29 are involved in the improvement of ventricular compliance promoted by aerobic exercise training in rats. Physiol Genomics 43: 665-673, 2011. First published March 29, 2011; doi:10.1152/physiolgenomics.00145.2010.-MiRNAs regulate cardiac development, hypertrophy, and angiogenesis, but their role in cardiac hypertrophy (CH) induced by aerobic training has not previously been studied. Aerobic training promotes physiological CH preserving cardiac function. This study assessed involvement of miRNAs-29 in CH of trained rats. Female Wistar rats (n = 7/group) were randomized into three groups: sedentary (S), training 1 (T1), training 2 (T2). T1: swimming sessions of 60 min/5 days/wk/10 wk. T2: similar to T1 until 8th wk. On the 9th wk rats swam 2x/day, and on the 10th wk 3x/day. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. We assessed: markers of training, CH by ratio of left ventricle (LV) weight/body wt and cardiomyocytes diameter, pathological markers of CH (ANF, skeletal alpha-actin, alpha/beta-MHC), collagen I and III (COLIAI and COLIIIAI) by real-time PCR, protein collagen by hydroxyproline (OH-proline) concentration, CF and CH by echocardiography. Training improved aerobic capacity and induced CH. MiRNAs-1, 133a, and 133b were downregulated as observed in pathological CH, however, without pathological markers. MiRNA-29c expression increased in T1 (52%) and T2 (123%), correlated with a decrease in COLIAI and COLIIIAI expression in T1 (27%, 38%) and T2 (33%, 48%), respectively. MiRNA-29c was inversely correlated to OH-proline concentration (r = 0.61, P = 0.05). The E/A ratio increased in T2, indicating improved LV compliance. Thus, these results show that aerobic training increase miR-29 expression and decreased collagen gene expression and concentration in the heart, which is relevant to the improved LV compliance and beneficial cardiac effects, associated with aerobic high performance training. (AU)