| Full text | |
| Author(s): |
Neto, Antonio M.
;
Valeriano, Maycom C.
;
Temperini, Marcia L. A.
;
Homem-de-Mello, Paula
;
Mamian-Lopez, Monica B.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY; v. 337, p. 8-pg., 2025-09-05. |
| Abstract | |
The halogenated C5-substituted uracil derivatives (5-fluor-, 5-chloro, and 5-bromouracil) have drawn attention recently due to their pharmacological uses, properties, and importance as biomarkers and water pollutants. From an analytical point of view, these species are expected to be at very low levels in biological and environmental samples, and the development of methodologies for their determination is a central goal in research. The Raman technique and one of its special effects, Surface-Enhanced Raman Scattering (SERS), is a very suitable approach for detecting and quantifying these compounds. In practice, enhancing Raman scattering requires a nano- structured noble metal surface with the species of interest attached to it. Still, to maximize the effect, a deeper comprehension of the nature of the analyte-metal surface interaction is desirable. The structural information SERS spectra provide can be complemented by theoretical approaches, such as the Density Functional Theory (DFT) calculations. This work studied three 5-halouracils attached to silver nanoparticles (AgNPs) from experimental and theoretical perspectives. The observed patterns in the spectroscopic behavior showed a trend related to the electronegativity at the halogenated moieties, suggesting their direct influence in enhancing C--C and C--O stretching modes. Then, the formation of base pairs with adenine through hydrogen bonding was studied as a strategy to improve the detectability through SERS, supported by the well-known high affinity of adenine towards metal nanoparticles. We show that adenine favors the orientation of the 5-halouracils, reaching an additional signal enhancement that is very useful for analytical purposes, as demonstrated for 5-FU, reaching a limit of detection (LOD) of 2.36 nmol L-1. Wavenumber shifts and intensification of N-H modes observed in the SERS spectra, along with DFT calculations, strongly suggest that forming hydrogen bonding (N-H--N) upon the interaction of the base pairs with an Ag20 cluster is key for improving the halouracils LOD through SERS. (AU) | |
| FAPESP's process: | 22/11983-4 - Spectroscopy signal enhancement: nanomaterials, theory, and computer simulation |
| Grantee: | Mauro Carlos Costa Ribeiro |
| Support Opportunities: | Research Projects - Thematic Grants |