Biochemical and cytogenetic consequences of settleable atmospheric particulate matter on Pacific white shrimp Litopenaeus vannamei

Author(s)

Copetti, F. ; Nobre, C. R. ; Paco, M. S. ; de Camargo, T. F. T. ; Moreno, B. B. ; Fernandes, M. N. ; Schveitzer, R. ; Pereira, C. D. S.

• Document type: Journal article
• Source: AQUATIC TOXICOLOGY; v. 283, p. 11-pg., 2025-06-01.

Abstract

This study hypothesizes that micro and nanoparticles of metals from metallurgical atmospheric emissions can trigger sublethal effects on the Pacific white shrimp Litopenaeus vannamei. We aimed to analyze cytotoxicity (Lysosomal Membrane Stability - LMS), Lipid peroxidation (LPO), genotoxicity (DNA strand break), and neurotoxicity (Acetylcholinesterase activity AChE) in shrimp exposed to environmentally relevant concentrations (0.001, 0.1, and 1.0 g.L- 1) of settleable atmospheric particulate matter (SePM) for different times (T2, T4, T7, T15 and T30 days), and in several tissues (gills, hemolymph, muscle,e and hepatopancreas). LPO within the first 2 day, and LMS showed significant differences. From the seventh to the fifteenth day of exposure, the concentration of 0.1 g.L- 1 exhibited significant effects. In the most extended exposure period (30 days), all concentrations triggered cytotoxicity effects on the Pacific white shrimp Litopenaeus vannamei. Thus, exposure to SePM can impair essential cellular functions, denoting a pre-pathological status. These findings underscore the potential hazards of metallurgical SePM to estuarine and farmed shrimp populations, emphasizing the need for ongoing monitoring and effective mitigation strategies to ensure aquaculture sustainability. (AU)

• FAPESP's process: 22/09868-2 - ATMOSPHERIC PARTICULATE MATERIAL AND ENVIRONMENTAL CONTAMINATION. IMPACT ASSESSMENT IN THE AQUATIC BIOTA IN AN INTEGRATED ECOPHYSIOTOXICOLOGICAL APPROACH
• Grantee: Marina de Souza Paço
• Support Opportunities: Scholarships in Brazil - Technical Training Program - Technical Training
• FAPESP's process: 22/10154-4 - Bioaccumulation, subcellular partition and biomarkers at different levels of biological organization of aquatic invertebrates exposed to metallic nanoparticles
• Grantee: Caio Rodrigues Nobre
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 22/06151-0 - Multi-user equipment approved in Grant 2019/08941-0: Microplate reader
• Grantee: Camilo Dias Seabra Pereira
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 19/08491-0 - Atmospheric particulate material and environmental contamination. Impact assessment in the aquatic biota in an integrated ecophysiotoxicological approach
• Grantee: Marisa Narciso Fernandes
• Support Opportunities: Research Projects - Thematic Grants