Atmospheric particulate material and environmental contamination. Impact assessment in the aquatic biota in an integrated ecophysiotoxicological approach

• Grant number: 19/08491-0
• Support Opportunities: Research Projects - Thematic Grants
• Start date: August 01, 2020
• End date: July 31, 2026
• Field of knowledge: Biological Sciences - Ecology
• Principal Investigator: Marisa Narciso Fernandes
• Grantee: Marisa Narciso Fernandes
• Host Institution: Centro de Ciências Biológicas e da Saúde (CCBS). Universidade Federal de São Carlos (UFSCAR). São Carlos , SP, Brazil
• City of the host institution: São Carlos
• Principal investigators: Camilo Dias Seabra Pereira ; Cléo Alcantara Costa Leite
• Associated researchers: cleoni dos santos carvalho ; Daniel Alberto Wunderlin ; Diana Amaral Monteiro ; Evelise Nunes Fragoso de Moura ; Fabiano Bendhack ; HELEN SADAUSKAS HENRIQUE ; Magdalena Victoria Monferrán ; Michael Elliott ; Silvia Tamie Matsumoto
• Associated research grant(s): 25/19454-9 - XXX Congress of the Brazilian Society for Microscopy and Microanalyses, AR.BR; 23/05129-3 - Setac Latin America 15th Biennial Meeting, AR.EXT; 23/00668-3 - Effect of temperature sublethal contamination by atmospheric particulate matter (APM), AP.R; + associated grants 23/01356-5 - Incorporation of metallic nanoparticles present in atmospheric particullate matter in tissues of Centropomus parallelus and Oreochromis niloticus, AP.R; 22/06151-0 - Multi-user equipment approved in Grant 2019/08941-0: Microplate reader, AP.EMU; 20/13975-3 - Multi-user Equipment approved in grant 20/08941-0: Ultracentrifuge, AP.EMU - associated grants
• Associated scholarship(s): 25/22357-5 - ATMOSPHERIC PARTICULATE MATERIAL AND ENVIRONMENTAL CONTAMINATION. IMPACT ASSESSMENT IN THE AQUATIC BIOTA IN AN INTEGRATED ECOPHYSIOTOXICOLOGICAL APPROACH, BP.TT; 25/20004-8 - Maintenance and ecotoxicological assays of aquatic vertebrates exposed to atmospheric particulate matter., BP.TT; 23/04265-0 - Atmospheric particulate matter and environmental contamination: Impact assessment for marine invertebrate of fisherie and aquaculture relevance, BP.DR; + associated scholarships 24/17923-9 - Study of the oyster shell, Crassostrea rhizophorae, as biological indicators for assessing environmental pollution by metals, BP.IC; 24/13340-9 - EVALUATION OF THE MUSSEL SHELL Perna leg AS AN INDICATOR OF ENVIRONMENTAL CONTAMINATION BY METALS/METALLOIDS AND METALLIC NANOPARTICLES, BP.IC; 24/12395-4 - Maintenance and ecotoxicological assays of aquatic vertebrates exposed to atmospheric particulate matter., BP.TT; 23/14486-4 - The use of relevant teleost models to characterize the effect of setteable atmospheric particulate matter (SePM) contamination, BP.PD; 24/00062-0 - Effect of temperature on histopathological damage caused by Settleable Atmospheric Particulate Matter (SePM), BP.IC; 23/12611-6 - n vitro biochemical and morphological analyzes in human lung epithelial cells (A549 lineage) exposed to atmospheric particulate matter (PM10)., BP.IC; 22/14646-9 - Plasticity, distribution and dynamics of ionocytes and cardiomyocytes: physiological mechanisms and adjustments to environmental contamination in different aquatic environments, BP.PD; 22/13445-0 - Maintenance and ecotoxicological assays of aquatic vertebrates exposed to atmospheric particulate matter., BP.TT; 22/10154-4 - Bioaccumulation, subcellular partition and biomarkers at different levels of biological organization of aquatic invertebrates exposed to metallic nanoparticles, BP.PD; 22/00987-9 - Evaluation of the effects of atmospheric particulate material on the species Macrobrachium rosenbergii oxidative system, BP.IC; 22/09868-2 - ATMOSPHERIC PARTICULATE MATERIAL AND ENVIRONMENTAL CONTAMINATION. IMPACT ASSESSMENT IN THE AQUATIC BIOTA IN AN INTEGRATED ECOPHYSIOTOXICOLOGICAL APPROACH, BP.TT; 21/11266-8 - Maintenance and ecotoxicological assays of aquatic invertebrates exposed to atmospheric particulate matter, BP.TT; 21/02906-3 - Plasticity, distribution and dynamics of ionocytes and cardiomyocytes: physiological mechanisms and adjustments to environmental contamination in different aquatic environments, BP.PD; 21/02245-7 - Maintenance and ecotoxicological assays of aquatic invertebrates exposed to atmospheric particulate matter, BP.TT; 21/02323-8 - Maintenance and ecotoxicological assays of aquatic vertebrates exposed to atmospheric particulate matter, BP.TT; 20/15253-5 - Bioaccumulation, subcellular partitioning and biomarkers at different levels of biological organization of aquatic invertebrates exposed to metallic nanoparticles, BP.PD - associated scholarships

Abstract

The industrial activity for iron (ore) producing and steel products processing results in the emission of particulate material in the atmosphere (APM) containing free metals and metallic nanoparticles. Many of these materials are emerging metals and nanoparticles that there are no environmental limits determined in the existing legislation. Dispersed in the atmosphere they may contaminate not only the aerial but also the aquatic environment and, passively, be incorporated by the local biota, including the humans. The accumulation of such metals and nanoparticles in the organisms may result in biochemical and physiological disfunctions impacting the homeostasis, metabolism and performance of animals and the health of the local population and the overall quality of life. This project proposes an integrated study concerns to constitution of APM, form and dynamic of cellular internalization, capacity and limit of metals and nanoparticles accumulation in different organs of aquatic organisms (vertebrates and invertebrates), bioavailability subcellular partition and possible damage to different biological processes. Physical and chemical studies will be done to identify and quantify the metals and metallic nanoparticles in the APM in one region recognised.by the magnitude of steel production and, ultra- and nanostructural morphological techniques will be applied to identify the materials at subcellular level, evaluate the crystallographic structure, oxidation state and bioavailability in the organs of animals exposed to APM in experiments, in vivo, ex vivo e in vitro. Genotoxic, biochemical, physiological and morphofunctional studies will be done in test-organisms to identify the mechanism of action of this mixture of this metallic contaminants and effects in the animal performance in the face of environmental challenges. The integration and range of his study for evaluating the toxic potential of APM, aquatic contamination and sublethal damages to the biota is innovative and highly relevant for future decisions regarding to this type of contamination and until now neglected by the environmental legislation (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ADORNO, HENRIQUE AIO; SOUZA, IARA DA COSTA; MONFERRAN, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; FERNANDES, MARISA NARCISO; MONTEIRO, DIANA AMARAL. A multi-biomarker approach to assess the sublethal effects of settleable atmospheric particulate matter from an industrial area on Nile tilapia (Oreochromis niloticus) br. Science of The Total Environment, v. 856, p. 13-pg., 2023-01-15. (19/08491-0)

SOUZA, IARA DA C.; MOROZESK, MARIANA; MANSANO, ADRISLAINE S.; MENDES, VITOR A. S.; AZEVEDO, VINICIUS C.; MATSUMOTO, SILVIA T.; ELLIOTT, MICHAEL; MONFERRAN, V, MAGDALENA; WUNDERLIN, DANIEL A.; FERNANDES, MARISA N.. Atmospheric particulate matter from an industrial area as a source of metal nanoparticle contamination in aquatic ecosystems. Science of The Total Environment, v. 753, JAN 20 2021. (17/03165-1, 19/08491-0)

SOUZA, IARA DA C.; MOROZESK, MARIANA; SIQUEIRA, PRISCILA; ZINI, ENZO; GALTER, IASMINI N.; MORAES, DANIEL A. DE; MATSUMOTO, SILVIA T.; WUNDERLIN, DANIEL A.; ELLIOTT, MICHAEL; FERNANDES, MARISA N.. etallic nanoparticle contamination from environmental atmospheric particulate matter in the last slab of the trophic chain: Nanocrystallography, subcellular localization and toxicity effect. Science of The Total Environment, v. 814, MAR 25 2022. (19/08491-0, 21/02906-3)

DE ANGELIS, C. F.; SOARES, M. P.; CARDOSO, I. L.; TAYLOR, E. W.; SADAUSKAS-HENRIQUE, H.; FRAGOSO-MOURA, E. N.; MILAN, J. A. M.; SOUZA, I. C.; WUNDERLIN, D. A.; MONFERRAN, M. V.; et al. Increased metabolic demand reveals morpho-functional impacts caused by settleable atmospheric particulate matter on fish gills. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, v. 112, p. 8-pg., 2024-11-26. (19/08491-0, 23/14486-4, 23/09904-1)

DA COSTA, REGIANE LUIZA; SOUZA, IARA COSTA; MOROZESK, MARIANA; DE CARVALHO, LUANA BESERRA; CARVALHO, CLEONI DOS SANTOS; MONFERRAN, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; FERNANDES, MARISA NARCISO; MONTEIRO, DIANA AMARAL. Toxic, genotoxic, mutagenic, and bioaccumulative effects of metal mixture from settleable particulate matter on American bullfrog tadpoles (Lithobates catesbeianus). Environmental Pollution, v. 340, p. 12-pg., 2023-11-09. (19/08491-0)

DE ANGELIS, C. F.; SOARES, M. P.; CARDOSO, I. L.; FILOGONIO, R.; TAYLOR, E. W.; MCKENZIE, D. J.; SOUZA, I. C.; WUNDERLIN, D. A.; MONFERRAN, M., V; FERNANDES, M. N.; et al. Settleable atmospheric particulate matter affects cardiorespiratory responses to hypoxia in Nile tilapia (Oreochromis niloticus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 257, p. 9-pg., 2022-04-22. (16/20158-6, 19/08491-0)

DUARTE, LUIS FELIPE DE ALMEIDA; ORTEGA, ANDRESSA DOS SANTOS BARBOSA; PACO, MARINA DE SOUZA; SADAUSKAS-HENRIQUE, HELEN; CESAR-RIBEIRO, CAIO; SOUZA, IARA COSTA; MONTEIRO, RAFAELLA; MONFERRAN, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; FERNANDES, MARISA NARCISO; et al. Settleable atmospheric particulate matter harms a marine invertebrate: Integrating chemical and biological damage in a bivalve model. Science of The Total Environment, v. 881, p. 10-pg., 2023-04-12. (22/09868-2, 19/08491-0, 20/15253-5, 21/02245-7)

SIQUEIRA, P. R.; DO CARMOO, TALITA LAURIE LUSTOSA; BONOMO, MARINA MARQUES; DOS SANTOS, FABRICIO APARECIDO; FERNANDES, MARISA NARCISO. Proliferative response avoids mutagenic effects of titanium dioxide (TiO2) nanoparticles in a zebrafish hepatocyte cell line. JOURNAL OF HAZARDOUS MATERIALS ADVANCES, v. 4, p. 11-pg., 2021-12-01. (19/08491-0)

MONTEIRO, RAFAELLA; SOUZA, IARA DA COSTA; MOROZESK, MARIANA; SOARES, MICHELLY PEREIRA; ANGELIS, CAROLINA FERNANDES DE; VIEIRA, NATHAN S.; BENDHACK, FABIANO; MONFERRAN, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; FERNANDES, MARISA NARCISO. Metalliferous atmospheric settleable particulate matter action on the fat snook fish (Centropomus parallelus): Metal bioaccumulation, antioxidant responses and histological changes in gills, hepatopancreas and kidneys.. Chemosphere, v. 330, p. 12-pg., 2023-04-27. (19/08491-0, 21/02906-3)

DE ANGELIS, C. F.; SOARES, M. P.; MCKENZIE, D. J.; TAYLOR, E. W.; CARDOSO, I. L.; SOUZA, I. C.; WUNDERLIN, D. A.; MONFERRAN, M. V.; FERNANDES, M. N.; LEITE, C. A. C.. Settleable atmospheric particulate matter affects the swimming performance and aerobic metabolic rate of Nile tilapia (Oreochromis niloticus). COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, v. 271, p. 8-pg., 2023-09-01. (19/08491-0)

FORTES, WILLIAM MANUEL PEREIRA ANTUNES; SOUZA, IARA DA COSTA; AZEVEDO, VINICIUS CAVICCHIOLI; GRIBOFF, JULIETA; MONFERRAN, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; MATSUMOTO, SILVIA TAMIE; FERNANDES, MARISA NARCISO. Metal/metalloid bioconcentration dynamics in fish and the risk to human health due to water contamination with atmospheric particulate matter from a metallurgical industrial area. Science of The Total Environment, v. 902, p. 13-pg., 2023-12-01. (19/08491-0)

GONZALEZ, MARIA PILAR; CORDERO-DE-CASTRO, ANDREA; SALVATIERRA, DAVID; KHOLSSI, RAJAA; FERNANDES, MARISA NARCISO; BLASCO, JULIAN; ARAUJO, CRISTIANO V. M.; PEREIRA, CAMILO DIAS SEABRA. Multi-level biological responses of Daphnia magna exposed to settleable atmospheric particulate matter from metallurgical industries. AQUATIC TOXICOLOGY, v. 263, p. 8-pg., 2023-10-01. (19/08491-0)

SOARES, MICHELLY PEREIRA; SILVA, LUDMILA MENDES; DE ANGELIS, CAROLINA FERNANDES; CARDOSO, ISRAEL LUZ; TAYLOR, EDWIN W.; SOUZA, IARA DA COSTA; BENDHACK, FABIANO; VIEIRA, NATHAN DE SOUZA; MONFERRAN, MAGDALENA V.; WUNDERLIN, DANIEL A.; et al. Effect of acute exposure to settleable atmospheric particulate matter emitted by the steel industry on hematology and innate immunity of fat snook (Centropomus parallelus). Marine Pollution Bulletin, v. 203, p. 13-pg., 2024-05-11. (19/08491-0, 23/09904-1)

COPETTI, F.; NOBRE, C. R.; PACO, M. S.; DE CAMARGO, T. F. T.; MORENO, B. B.; FERNANDES, M. N.; SCHVEITZER, R.; PEREIRA, C. D. S.. Biochemical and cytogenetic consequences of settleable atmospheric particulate matter on Pacific white shrimp Litopenaeus vannamei. AQUATIC TOXICOLOGY, v. 283, p. 11-pg., 2025-06-01. (22/09868-2, 22/10154-4, 22/06151-0, 19/08491-0)

NOBRE, CAIO RODRIGUES; PACO, MARINA DE SOUZA; DUARTE, LUIS FELIPE DE ALMEIDA; ORTEGA, ANDRESSA DOS SANTOS BARBOSA; MORENO, BEATRIZ BARBOSA; DE CAMARGO, THIAGO FELICISSIMO TURIBIO; PARREIRA, LETICIA MALVESTIO; SOUZA, IARA DA COSTA; MONFERR, MAGDALENA VICTORIA; WUNDERLIN, DANIEL ALBERTO; et al. Systemic effects of settleable atmospheric particulate matter (SePM) on swamp ghost crab Ucides cordatus. Science of The Total Environment, v. 938, p. 10-pg., 2024-05-30. (19/08491-0, 22/10154-4, 22/09868-2)

CARVALHO, LUANA B.; SOUZA, IARA C.; SOARES, MICHELLY P.; MONFERRAN, MAGDALENA, V; WUNDERLIN, DANIEL A.; MATSUMOTO, SILVIA T.; FERNANDES, MARISA N.. Metal/metalloid concentrations and multi-biomarkers in blood of Nile tilapia living in coastal lagoons near metallurgical industrial areas: an integrated evaluation. Environmental Toxicology and Chemistry, v. N/A, p. 16-pg., 2025-04-17. (19/08491-0, 23/01356-5)

CARVALHO, MARIANA V.; MONTEIRO, RAFAELLA; SOUZA, IARA DA C.; GRIBOFF, JULIETA; BENDHACK, FABIANO; MONFERRAN, MAGDALENA V.; WUNDERLIN, DANIEL A.; FERNANDES, MARISA N.. Muscle oxidative stress, neurotoxicity and human health risks from consuming fish exposed to metallic settleable atmospheric particulate matter. Marine Pollution Bulletin, v. 214, p. 15-pg., 2025-03-14. (19/08491-0, 23/01356-5)

DUARTE, LUIS FELIPE DE ALMEIDA; ORTEGA, ANDRESSA DOS SANTOS BARBOSA; NOBRE, CAIO RODRIGUES; SADAUSKAS-HENRIQUE, HELEN; GUSSO-CHOUERI, PALOMA KACHEL; SOUZA, IARA COSTA; CARVALHO, MARIANA, V; WUNDERLIN, DANIEL ALBERTO; FERNANDES, MARISA NARCISO; PEREIRA, CAMILO DIAS SEABRA. Coastal fallout of settleable particulate matter: Metal bioaccumulation and sublethal toxicity in estuarine bivalves with implications for human health. Marine Pollution Bulletin, v. 220, p. 17-pg., 2025-07-17. (22/09868-2, 19/08491-0, 22/10154-4, 21/02245-7, 20/15253-5, 22/06151-0)