Interferon-induced ADP-ribosylation: technical developments driving ICAB discovery

Author(s)

Ribeiro, Victoria Chaves ; Russo, Lilian Cristina ; Dure, Dulce Maria Gonzalez ; Hoch, Nicolas Carlos

• Document type: Journal article
• Source: BIOSCIENCE REPORTS; v. 45, n. 3, p. 13-pg., 2025-03-01.

Abstract

Cells respond to a variety of internal and external stimuli by regulating the activities of different signal- ling cascades and cellular processes, often via chemical modifications of biological macromolecules that modulate their overall levels, biochemical activities or biophysical interactions. One such modification, termed ADP-ribosylation (ADPr), is emerging as an important player in the interferon (IFN) response, but the molecular targets and functions of ADP-ribosyltransferases within this core component of innate immunity still remains unclear. We and others have recently identified that stimulation of IFN signalling cascades promotes the formation of a novel cytosolic structure in human cells that is enriched in ADP-ribosyl modifications. Here, we propose to name these structures 'interferon-induced cytosolic ADPr bodies' (ICABs) and discuss their known components and potential functions. We also review methods to detect ICABs (and cellular ADPr in general) using a range of recently developed reagents. This lays the foundation for future studies aimed at elucidating the molecular functions of ICABs and ADPr in innate immune responses, which is a central unanswered question in the field. (AU)

• FAPESP's process: 20/05317-6 - Inhibition of the viral macrodomain as a strategy for Coronavirus treatment
• Grantee: Nicolas Carlos Hoch
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/18007-5 - Protein ADP-ribosylation: DNA damage signalling and impacts on human health
• Grantee: Nicolas Carlos Hoch
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 23/15157-4 - Cellular roles of the PARP9/DTX3L heterodimer
• Grantee: Victoria Chaves Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/25914-1 - Role of PARP9/DTX3L-dependent H4K91 ubiquitination in the DNA damage response
• Grantee: Victoria Chaves Ribeiro
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 19/06039-2 - EMU awarded in the process 2018/18007-5: TissueFAXS microscope
• Grantee: Nicolas Carlos Hoch
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 22/10783-1 - Recruitment and activation of the PARP9-DTX3L heterodimer in response to DNA damage
• Grantee: Dulce María González Duré
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 20/11162-5 - Development of a cellular assay to measure SARS-CoV-2 Nsp3 macrodomain activity in vitro
• Grantee: Lilian Cristina Russo Vieira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral