Acute toxicity and histopathological effects of pyriproxyfen in adult male and female zebrafish (Danio rerio)

Author(s)

Gameiro, Beatriz G. ; Brito, Rafaella S. ; Valle, Amanda de A. ; Tominaga, Flavio K. ; Kummrow, Fabio ; Giannocco, Gisele ; Pitol, Dimitrius Leonardo ; Dsouki, Nuha A. ; Maciel, Rui M. de B. ; Pereira, Bruno F.

• Document type: Journal article
• Source: ENVIRONMENTAL SCIENCE-ADVANCES; v. 4, n. 7, p. 12-pg., 2025-05-15.

Abstract

Pyriproxyfen (PPF) is a larvicide used to combat and control insects in agriculture, veterinary medicine and public health, particularly targeting vectors such as mosquitoes that transmit dengue fever. Despite being generally considered toxic to non-target organisms, the toxic effects of this substance have already been described. In this study, we investigated the effects of acute exposure (96 h) on the mortality and histopathology of different organs: the liver, gills, intestine, kidneys and gonads of adult individuals of the species Danio rerio. The results showed that the solubility of PPF prevents the accurate determination of LC50-96 h; however, despite causing slight toxicity to female gills and gonads, the substance presented deleterious effects on the other tissues analyzed. We conclude that PPF proved to be toxic even in acute exposures; however, more studies with this substance should be expanded to chronic experiments. (AU)

• FAPESP's process: 22/07845-5 - Toxic effects of pyriproxyfen on liver, gills and gonads morphology of Danio rerio
• Grantee: Beatriz Gomes Gameiro
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 22/03094-5 - Project Zebratox: toxicological screening of larvicides of public and veterinary health importance
• Grantee: Bruno Fiorelini Pereira
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 24/01418-3 - JOINT EFFECTS OF MICROPLASTIC AND INSECTICIDES APPLIED IN PUBLIC HEALTH CAMPAIGNS AND VETERINARY MEDICINE ON DIFFERENT STAGES OF DEVELOPMENT SPECIES DANIO RERIO (ZEBRAFISH).
• Grantee: Bruno Fiorelini Pereira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/15540-2 - TOXICOLOGICAL EVALUATION OF DIFFERENT FORMULATIONS OF DIFLUBENZURON: ANALYSIS OF MULTIPLE MORPHOLOGICAL BIOMARKERS IN ZEBRAFISH (Danio rerio)
• Grantee: Amanda de Azevedo Valle
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 24/10993-1 - Joint effects of microplastic and insecticides applied in public health campaigns and veterinary medicine on different stages of development species danio rerio (zebrafish)
• Grantee: Flavio Kiyoshi Tominaga
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• FAPESP's process: 23/09884-0 - Analysis of histopathological effects and endocrine disruption in chronic exposure to Diflubenzuron and Piriproxyfen in the experimental model Danio rerio
• Grantee: Rafaella da Silva Brito
• Support Opportunities: Scholarships in Brazil - Doctorate