Comparative analysis of the effects of the biopesticide Azadirachtin and the synthetic insecticide Piriproxifem on the testes of Ceraeochrysa claveri (Navas, 1911) (Neuroptera: Chrysopidae). A morphological, ultrastructural and molecular approach.

• Grant number: 17/09901-1
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: December 01, 2017
• End date: May 31, 2021
• Field of knowledge: Agronomical Sciences - Agronomy - Plant Health
• Principal Investigator: Daniela Carvalho dos Santos
• Grantee: Ana Silvia Gimenes Garcia
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Insects of the Chrysopidae family are natural enemies used in biological control, being an important tool in organic agriculture and integrated pest management. In addition to biological pest control, the use of natural insecticides has been gaining prominence, as they are less harmful to man and the environment, but the use of synthetic insecticides is still very high, interfering with the life cycle of natural enemies, and consequently, on the biological control of pests. In order to evaluate the effect of azadirachtin, a compound extracted from the neem tree with biopesticidal properties, and the synthetic insecticide pyriproxyfen on adult male chrysopids, Diatraea saccharalis eggs will be treated with these compounds at concentrations 12 and 36 mg ia/L for azadirachtin (AzaMax® commercial formulation), and 50 and 100 mg ia/L for pyriproxyfen (commercial formulation Tiger 100 EC®) and will be offered as food to larvae of Ceraeochrysa claveri. Adult testes will be evaluated through cytochemical, immunofluorescent, ultrastructural and bioinformatic (transcriptome) techniques. In addition, will bem evaluated the effect of these compounds on the fecundity of females and fertility of the eggs from the cross between untreated females and treated males. Through the knowledge of these organs it will be possible to compare the effect of a natural insecticide and a synthetic one on the same organ of a natural enemy and to evaluate the effects that they will cause.

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

GARCIA, Ana Silvia Gimenes. Comparative analysis of the effects of the biopesticide Azadirachtin and the synthetic insecticide Piriproxifem on the testes of Ceraeochrysa claveri (Navas, 1911) (Neuroptera: Chrysopidae). A morphological, ultrastructural and molecular approach. 2021. Doctoral Thesis - Universidade Estadual Paulista (Unesp). Instituto de Biociências. Botucatu Botucatu.