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Toxicity of neem (Azadirachta indica A. Juss) on spermatogenesis of Ceraeochrysa claveri (Navás, 1911) (Neuroptera: Chrysopidae): study cytochemical, immunocytochemical and ultrastructural

Grant number: 12/02879-7
Support type:Scholarships in Brazil - Master
Effective date (Start): August 01, 2012
Effective date (End): February 28, 2014
Field of knowledge:Agronomical Sciences - Agronomy - Plant Health
Principal researcher:Daniela Carvalho dos Santos
Grantee:Ana Silvia Gimenes Garcia
Home Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil


The search for the preservation of natural resources is currently sensitizing the society about the importance of food, requiring products with minimal use of insecticides that are harmful to health, and to increase the supply of safe food to this population, we see growing use of agriculture organic and integrated pest management. In search of insects that can be used in biological control, which is an important tool in organic farming and integrated pest management, the lacewings is proving a strong controlling agent, since their larvae are predators of eggs and larvae of various insects pests of different crops. In order to evaluate the effect of neem oil, a plant extract with properties insecticides on lacewings, eggs of Diatraea saccharalis will be treated with neem oil at three different concentrations and offered as food to larvae of Ceraeochrysa claveri, thereby evaluating the effect of ingestion of neem oil in the larval stage of the spermatogenic lineage cells from the testes of third instar larva, pupa and adult of C.claveri. Through knowledge of the morphology and ultrastructure of the testes, as well as the process of spermatogenesis and the effect of ingestion of neem oil on this organ, we can inform the future viability of the combined use of neem oil and lacewings in cultures that emphasize maintenance of diversity and integrated pest management.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GIMENES GARCIA, ANA SILVIA; SCUDELER, ELTON LUIZ; FELIPE PINHEIRO, PATRICIA FERNANDA; DOS SANTOS, DANIELA CARVALHO. Can exposure to neem oil affect the spermatogenesis of predator Ceraeochrysa claveri?. PROTOPLASMA, v. 256, n. 3, p. 693-701, MAY 2019. Web of Science Citations: 2.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GARCIA, Ana Silvia Gimenes. Toxicidade do óleo de nim (Azadirachta indica A. JUSS) na espermatogênese de Ceraeochrysa claveru (NAVÁS, 1911)(Neuroptera: Chysopidae) : estudo citoquímico, imunocitoquímico e ultraestrutural. 2014. 86 f. Master's Dissertation - Universidade Estadual Paulista "Júlio de Mesquita Filho" Instituto de Biociências (Campus de Botucatu)..

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